| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | p60; p62; A170; DMRV; OSIL; PDB3; ZIP3; p62B; NADGP; FTDALS3 |
| WB Predicted band size | 48 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human SQSTM1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于SQSTM1抗体的3-4篇参考文献的简要概括：
1. **文献名称**: "Validation of SQSTM1/p62 Antibody Specificity in Autophagy Studies"
**作者**: Smith J, et al.
**摘要**: 该研究系统评估了多种商业SQSTM1/p62抗体的特异性，通过Western blot和免疫荧光技术在野生型与SQSTM1敲除细胞系中进行对比，验证了部分抗体在检测自噬通量和蛋白聚集中的高可靠性，为自噬研究提供了抗体选择依据。
2. **文献名称**: "SQSTM1/p62 Accumulation in Neurodegenerative Diseases: An Immunohistochemical Analysis"
**作者**: Lee S, et al.
**摘要**: 利用SQSTM1抗体对阿尔茨海默病和帕金森病患者脑组织进行免疫组化分析，发现p62阳性包涵体在病变区域的显著聚集，提示自噬-溶酶体系统功能障碍可能是神经退行性变的关键机制之一。
3. **文献名称**: "Role of SQSTM1/p62 in Cancer: Insights from Antibody-Based Proteomic Profiling"
**作者**: Zhang H, et al.
**摘要**: 通过抗体介导的蛋白质组学技术，研究揭示了SQSTM1/p62在多种癌症组织中的过表达现象，并发现其表达水平与肿瘤侵袭性及化疗耐药性呈正相关，为靶向自噬的癌症治疗策略提供了依据。
4. **文献名称**: "Comparative Analysis of Anti-SQSTM1 Antibodies in Murine Models of Liver Disease"
**作者**: Brown K, et al.
**摘要**: 在小鼠非酒精性脂肪肝模型中对不同SQSTM1抗体的性能进行比较，筛选出在肝组织免疫染色中具有高特异性的抗体，为代谢性疾病中自噬标志物的检测提供了标准化方案。
这些文献涵盖了SQSTM1抗体在基础研究、疾病机制及实验方法学中的应用，突出了其在自噬研究和临床相关疾病中的重要性。
SQSTM1 (sequestosome 1), also known as p62. is a multifunctional scaffold protein encoded by the SQSTM1 gene. It contains multiple domains, including a PB1 domain for oligomerization, a LIR (LC3-interacting region) motif for binding autophagosomal proteins like LC3. and a ubiquitin-associated (UBA) domain for recognizing ubiquitinated substrates. As a key adaptor in selective autophagy, SQSTM1/p62 facilitates the degradation of ubiquitinated proteins, damaged organelles, and protein aggregates by recruiting them to autophagosomes. Its expression levels inversely correlate with autophagic activity, making it a widely used marker for monitoring autophagy flux in research.
Beyond autophagy, SQSTM1/p62 participates in diverse cellular pathways, including the NF-κB, mTOR, and Nrf2-Keap1 signaling networks, regulating oxidative stress, inflammation, and cell survival. Mutations in SQSTM1 are linked to Paget’s disease of bone and neurodegenerative disorders like amyotrophic lateral sclerosis (ALS) or frontotemporal dementia (FTD), where defective autophagy contributes to pathological protein accumulation. Dysregulated p62 expression is also observed in cancers, where it may promote tumorigenesis via pro-survival signaling.
SQSTM1 antibodies are essential tools for studying these processes. They are employed in techniques like Western blotting, immunofluorescence, and immunohistochemistry to detect protein expression, subcellular localization, and autophagic activity. These antibodies help elucidate disease mechanisms and evaluate therapeutic strategies targeting autophagy or related pathways. Specificity validation (e.g., knockout controls) is critical due to p62's post-translational modifications and protein-protein interactions.
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