首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人神经节苷脂GM2活化剂(GM2A)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、尿液、组织匀浆、细胞裂解物样本中的GM2A含量。GM2A 是一个重要靶点，它与 GM2 神经节苷脂代谢相关。其研究机制主要围绕在辅助降解 GM2 神经节苷脂上。若 GM2A 功能异常，会导致 GM2 神经节苷脂蓄积，引发神经系统疾病，故围绕其开展的研究对相关疾病治疗意义重大。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，本试剂盒适用于生命科学领域的基础研究，例如探究脂质代谢异常相关疾病的分子机制、评估疾病模型中GM2A的表达动态变化，或用于神经生物学、细胞生物学实验中分析特定干预条件对GM2A蛋白水平的影响，为研究该靶点在生理病理过程中的作用提供定量化检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Cerebroside sulfate activator protein ELISA Kit; ganglioside GM2 activator ELISA Kit; Ganglioside GM2 activator isoform short ELISA Kit; Ganglioside GM2 activator precursor ELISA Kit; GM2 activator ELISA Kit; GM2 AP ELISA Kit; GM2 ganglioside activator ELISA Kit; GM2 ganglioside activator protein ELISA Kit; GM2-AP ELISA Kit; GM2A ELISA Kit; GM2AP ELISA Kit; OTTHUMP00000160619 ELISA Kit; SAP 3 ELISA Kit; SAP-3 ELISA Kit; SAP3 ELISA Kit; SAP3_HUMAN ELISA Kit; Shingolipid activator protein 3 ELISA Kit; Sphingolipid activator protein 3 ELISA Kit
缩写：GM2A
Uniprot No.：P17900
种属：Homo sapiens (Human)
样本类型：serum, plasma, urine, tissue homogenates, cell lysates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human GM2A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 84-95 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 87-99 | |||||
| 1:4 | Average % | 100 | ||||
| Range % | 95-106 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 92-102 | |||||
回收率：
| The recovery of human GM2A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 90-103 | ||||
| EDTA plasma (n=4) | 90 | 84-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.0432.0502.0471.9215001.6191.6841.6521.5262501.1051.2001.1531.0271250.6830.7030.6930.56762.50.4030.4210.4120.28631.20.2960.3220.3090.18315.60.2410.2350.2380.11200.1230.1290.126 | ||||||
货期：3-5 working days
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