首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠胰高血糖素样肽1(Glp-1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的Glp1含量。Glp1即胰高血糖素样肽-1，是肠道L细胞分泌的一种肽类激素。其背景在于可延缓胃排空、抑制食欲等。研究机制上，它能与相应受体结合，刺激胰岛素分泌，降低血糖，同时还参与能量代谢调控，目前是糖尿病、肥胖症等疾病药物研发热门靶点。试剂盒检测范围为0.625 pg/mL-40 pg/mL，该产品适用于糖尿病机制研究、代谢性疾病模型评估、肠道内分泌功能分析及药物开发中Glp - 1相关调控效应的体外验证，为探索胃肠激素信号通路提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：Glp1
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.625 pg/mL-40 pg/mL
灵敏度：0.156 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat GLP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 86 | ||||
| Range % | 84-95 | |||||
| 1:200 | Average % | 95 | ||||
| Range % | 91-100 | |||||
| 1:400 | Average % | 84 | ||||
| Range % | 81-89 | |||||
| 1:800 | Average % | 92 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of rat GLP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 87-96 | ||||
| EDTA plasma (n=4) | 103 | 95-108 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected402.6072.7442.6762.531202.3032.4512.3772.232101.8171.9221.8701.72551.1331.1911.1621.0172.50.6210.6320.6270.4821.250.3440.3550.3500.2050.6250.2340.2480.2410.09600.1400.1500.145 | ||||||
货期：3-5 working days
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