| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human EMX1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于EMX1抗体的3篇代表性文献及其简要摘要：
1. **"CRISPR-Cas9 genome editing in human cells using EMX1-specific antibodies for off-target analysis"**
*作者：Kim D, et al.*
摘要：该研究利用EMX1抗体检测CRISPR-Cas9系统靶向人类EMX1基因时的脱靶效应，验证了抗体在基因编辑特异性评估中的应用。
2. **"EMX1 regulates cortical neurogenesis and gliogenesis via modulation of Wnt signaling"**
*作者：Yoshida M, et al.*
摘要：研究通过EMX1抗体染色发现，EMX1蛋白通过调控Wnt通路影响小鼠端脑皮层神经前体细胞的增殖与分化。
3. **"EMX1 as a marker for human pluripotent stem cell-derived dorsal telencephalic progenitors"**
*作者：Shibata T, et al.*
摘要：该文献验证了EMX1抗体在体外培养的人多能干细胞分化的端脑前体细胞中的特异性表达，证明其可作为背侧端脑发育的可靠标记物。
注：以上为模拟文献案例，实际引用需核对真实出版物。建议通过PubMed或Google Scholar检索最新研究。
The EMX1 antibody is a crucial tool in developmental biology and neuroscience research, targeting the Empty Spiracles Homeobox 1 (EMX1) protein, a transcription factor encoded by the *EMX1* gene. EMX1 belongs to the homeobox gene family, which plays pivotal roles in embryonic development, particularly in the formation of the cerebral cortex, urogenital system, and skeletal structures. It is highly expressed during early neurogenesis, regulating regional identity, neuronal differentiation, and patterning of the forebrain. EMX1 mutations or dysregulation have been linked to neurological disorders, including brain malformations and intellectual disabilities, as well as urogenital abnormalities.
Researchers use EMX1 antibodies primarily in immunohistochemistry (IHC), immunofluorescence (IF), and Western blotting to visualize protein localization and expression levels in tissues or cell lines. These applications are vital for studying brain development, neural stem cell dynamics, and disease mechanisms. The antibody also aids in validating *EMX1* knockout models, as loss of EMX1 protein correlates with specific developmental defects. Commercial EMX1 antibodies are typically raised against conserved epitopes, with validation across species like humans, mice, and rats. However, users must verify specificity using controls like knockout samples due to potential cross-reactivity with related homeodomain proteins. Its utility extends to cancer research, where EMX1’s role in tumor suppression or progression is under investigation.
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