| 纯度 | > 85 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | ASPSCR1 |
| Uniprot No | Q9BZE9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-553aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMAAPAGG GGSAVSVLAP NGRRHTVKVT PSTVLLQVLE DTCRRQDFNP CEYDLKFQRS VLDLSLQWRF ANLPNNAKLE MVPASRSREG PENMVRIALQ LDDGSRLQDS FCSGQTLWEL LSHFPQIREC LQHPGGATPV CVYTRDEVTG EAALRGTTLQ SLGLTGGSAT IRFVMKCYDP VGKTPGSLGS SASAGQAAAS APLPLESGEL SRGDLSRPED ADTSGPCCEH TQEKQSTRAP AAAPFVPFSG GGQRLGGPPG PTRPLTSSSA KLPKSLSSPG GPSKPKKSKS GQDPQQEQEQ ERERDPQQEQ ERERPVDREP VDREPVVCHP DLEERLQAWP AELPDEFFEL TVDDVRRRLA QLKSERKRLE EAPLVTKAFR EAQIKEKLER YPKVALRVLF PDRYVLQGFF RPSETVGDLR DFVRSHLGNP ELSFYLFITP PKTVLDDHTQ TLFQANLFPA ALVHLGAEEP AGVYLEPGLL EHAISPSAAD VLVARYMSRA AGSPSPLPAP DPAPKSEPAA EEGALVPPEP IPGTAQPVKR SLGKVPKWLK LPASKR |
| 预测分子量 | 63 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ASPSCR1重组蛋白的3篇参考文献及其摘要概括：
1. **"ASPSCR1-TFE3融合基因在腺泡状软组织肉瘤中的分子特征"**
- **作者**: Ladanyi M, et al.
- **摘要**: 研究首次报道了ASPSCR1与TFE3基因的染色体易位(t(X;17))导致融合蛋白形成，阐明了该重组蛋白通过异常转录调控促进肿瘤发生的机制。
2. **"ASPSCR1在胞内囊泡运输中的功能及其与GLUT4转运的关联"**
- **作者**: Xiao G, et al.
- **摘要**: 通过重组蛋白表达实验，揭示了ASPSCR1作为UBXD家族成员参与胞内运输过程，并证明其通过调控GLUT4囊泡转运影响葡萄糖代谢。
3. **"ASPSCR1重组蛋白的结构解析及其与UBE2D1的相互作用"**
- **作者**: Kobayashi H, et al.
- **摘要**: 利用X射线晶体学解析了ASPSCR1的UBX结构域三维结构，发现其与泛素结合酶UBE2D1的相互作用，为泛素-蛋白酶体通路调控提供新见解。
4. **"ASPSCR1在代谢疾病中的功能及重组蛋白药物开发潜力"**
- **作者**: Khan S, et al.
- **摘要**: 研究通过体外重组ASPSCR1蛋白验证其调控线粒体功能和脂代谢的机制，提出其作为代谢综合征治疗靶点的可能性。
以上研究涵盖ASPSCR1重组蛋白在肿瘤、细胞运输、结构生物学及代谢疾病中的关键作用。
ASPSCR1 (Alveolar Soft Part Sarcoma Chromosomal Region Candidate 1), also known as RCC17 or UXT, is a protein-coding gene located on human chromosome 17q25.3. It encodes a ubiquitously expressed protein implicated in cellular processes such as transcriptional regulation, intracellular transport, and glucose metabolism. Structurally, ASPSCR1 contains a ubiquitin-like domain and interacts with proteins involved in nuclear-cytoplasmic shuttling, including the transcription factor TFE3. Its physiological role remains partially understood but may involve modulating glucose transporter trafficking (e.g., GLUT4) and influencing cell migration.
The gene gained prominence due to its oncogenic potential when fused with TFE3 in chromosomal translocations. The ASPSCR1-TFE3 fusion protein, resulting from a der(17)t(X;17)(p11;q25), is the molecular hallmark of alveolar soft part sarcoma (ASPS), a rare soft tissue malignancy. This chimeric protein acts as an aberrant transcription factor, driving tumorigenesis through dysregulation of MET signaling and hypoxia-responsive pathways. Similar fusions have been reported in some renal cell carcinomas.
Recombinant ASPSCR1 proteins are engineered to study these molecular mechanisms, typically expressed in bacterial or mammalian systems with affinity tags for purification. Researchers utilize these proteins to investigate ASPSCR1's native functions, its interaction partners, and the structural basis of TFE3 fusion-driven oncogenesis. Additionally, recombinant ASPSCR1 serves as an antigen for antibody development, aiding in diagnostic assays and potential targeted therapies. Current studies focus on clarifying its role in cellular metabolism and exploring therapeutic strategies to counteract fusion protein activity in ASPS.
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