首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠神经调制蛋白(GAP43)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、细胞裂解物、组织匀浆样本中的GAP43含量。GAP43 即生长相关蛋白 43，是神经系统发育和再生过程中的重要蛋白。它参与轴突生长、突触可塑性等过程。研究发现，它在神经损伤修复、学习记忆等机制中发挥作用，其表达变化与神经系统疾病发展密切相关，有望成治疗靶点。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于神经科学领域的基础研究，如神经退行性疾病模型构建、创伤后神经再生机制探索以及体外神经元培养实验中GAP43动态变化的定量分析。为神经系统相关疾病的分子机制研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Gap43 ELISA Kit; Neuromodulin ELISA Kit; Axonal membrane protein GAP-43 ELISA Kit; Growth-associated protein 43 ELISA Kit; Protein F1 ELISA Kit
缩写：GAP43
Uniprot No.：P07936
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell lysates, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat GAP43 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 98 | ||||
| Range % | 90-102 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 98-108 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 88-99 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 91-103 | |||||
回收率：
| The recovery of rat GAP43 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 94-102 | ||||
| EDTA plasma (n=4) | 94 | 90-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.1072.2762.1922.037101.5131.6161.5651.41050.9550.9230.9390.7842.50.5460.5570.5520.3971.250.3890.3720.3810.2260.6250.2710.2860.2790.1240.3120.1910.1980.1950.04000.1540.1560.155 | ||||||
货期：3-5 working days
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