首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠游离睾酮(F-TESTO)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆样本中的F-TESTO含量。试剂盒检测范围为0.3 pg/mL-60 pg/mL，该产品可为生殖生物学研究、环境内分泌干扰物评估、睾丸功能障碍模型验证以及雄激素相关药物作用机制探索提供可靠数据支持，广泛应用于神经内分泌调控、性腺衰老、代谢综合征等动物实验的激素水平检测，为科研工作者提供标准化的睾酮活性状态分析工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：F-TESTO
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma
检测范围：0.3 pg/mL-60 pg/mL
灵敏度：0.15 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat F-TESTO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 90-101 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 89-99 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 90-100 | |||||
回收率：
| The recovery of rat F-TESTO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 89-104 | ||||
| EDTA plasma (n=4) | 94 | 88-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 600.2020.2050.204 150.3590.3420.351 4.80.6100.6230.617 1.21.0071.0121.010 0.31.4641.4571.461 02.4392.4542.447 | ||||||
货期：3-5 working days
×
