首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠卵泡抑素(FS)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FST含量。试剂盒检测范围为0.2 ng/mL-40 ng/mL，适用于卵巢功能研究、生殖内分泌调控机制探索、疾病模型FS表达动态分析等科研领域。通过优化样本预处理流程，可兼容高脂血浆及多种组织匀浆的检测需求，为体外实验提供稳定可靠的数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：FstFollistatin ELISA Kit; FS ELISA Kit; Activin-binding protein ELISA Kit
缩写：FST
Uniprot No.：P21674
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：0.2 ng/mL-40 ng/mL
灵敏度：0.1 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat FS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 84 | ||||
| Range % | 81-89 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 92-98 | |||||
| 1:4 | Average % | 102 | ||||
| Range % | 98-106 | |||||
| 1:8 | Average % | 89 | ||||
| Range % | 84-93 | |||||
回收率：
| The recovery of rat FS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 89-95 | ||||
| EDTA plasma (n=4) | 89 | 85-93 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average400.1670.1600.164100.4010.4320.4173.20.5490.5360.5430.80.9510.9420.9470.21.4851.4781.48202.5482.4092.479 | ||||||
货期：3-5 working days
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