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产品描述：小鼠纤维连接蛋白Ⅲ型结构域蛋白5(FNDC5)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FNDC5含量。FNDC5，也称为Irisin的前体，是一种肌肉因子，主要在骨骼肌表达。其通过蛋白水解酶作用释放Irisin，调节糖脂代谢和心血管稳态。研究显示，FNDC5可影响细胞增殖与迁移，具有抑制平滑肌细胞增殖与迁移的作用。研究机制涉及FNDC5与不同受体的结合，以及其分泌产物Irisin的生理功能。试剂盒检测范围为6.25 pg/mL-400 pg/mL，适用于探究FNDC5在代谢调节、运动生理及能量平衡等领域的分子机制研究，通过血清/血浆样本可检测循环系统中FNDC5的动态变化，而组织匀浆检测则适用于肌肉、脂肪等组织的局部表达分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Fndc5 ELISA kit; Frcp2 ELISA kit; PepFibronectin type III domain-containing protein 5 ELISA kit; Fibronectin type III repeat-containing protein 2 ELISA kit; Peroxisomal protein ELISA kit; PeP) [Cleaved into: Irisin] ELISA kit
缩写：FNDC5
Uniprot No.：Q8K4Z2
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：6.25 pg/mL-400 pg/mL
灵敏度：1.56 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse FNDC5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 86-95 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 92-102 | |||||
| 1:4 | Average % | 88 | ||||
| Range % | 86-93 | |||||
| 1:8 | Average % | 89 | ||||
| Range % | 84-94 | |||||
回收率：
| The recovery of mouse FNDC5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 88-96 | ||||
| EDTA plasma (n=4) | 100 | 95-103 | ||||
货期：3-5 working days
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