首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠纤连蛋白(FN)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的FN1含量。FN1，即纤连蛋白1，是一种存在于血浆和细胞外基质中的糖蛋白，参与细胞粘附和迁移等过程。研究显示，FN1在运动中通过激活肝脏自噬和改善胰岛素敏感性来促进机体健康，其具体机制涉及骨骼肌分泌FN1与肝脏α5β1整合素受体结合，进而调节肝脏自噬和胰岛素敏感性。试剂盒检测范围为0.78 ng/mL-50 ng/mL，适用于体外培养细胞分泌功能评估、组织工程模型基质成分分析，以及心血管疾病、纤维化疾病等动物模型中细胞外基质动态变化的科研探索，助力于细胞行为机制、病理生理过程及相关药物筛选等基础研究领域的数据获取。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Fn1Fibronectin ELISA kit; FN) [Cleaved into: Anastellin] ELISA kit
缩写：FN1
Uniprot No.：P04937
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.78 ng/mL-50 ng/mL
灵敏度：0.195 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat FN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1000 | Average % | 96 | ||||
| Range % | 85-100 | |||||
| 1:2000 | Average % | 87 | ||||
| Range % | 82-93 | |||||
| 1:4000 | Average % | 98 | ||||
| Range % | 95-100 | |||||
| 1:8000 | Average % | 92 | ||||
| Range % | 88-94 | |||||
回收率：
| The recovery of rat FN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 87-98 | ||||
| EDTA plasma (n=4) | 99 | 89-109 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected502.3702.4052.3882.245252.0152.0632.0391.89612.51.6131.6271.6201.4776.251.1151.1361.1260.9833.120.6850.6790.6820.5391.560.4320.4670.4500.3070.780.2780.2910.2850.14200.1420.1440.143 | ||||||
货期：3-5 working days
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