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产品描述：人脂筏标记蛋白1(FLOT1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的FLOT1含量。FLOT1 作为靶点，它在细胞生物学等领域有着重要背景，是细胞膜微囊相关蛋白。在研究机制方面，其可能参与细胞黏附、信号转导等过程，与肿瘤细胞的增殖、迁移、侵袭等活动存在关联，是当前肿瘤等疾病研究的潜在方向。试剂盒检测范围为12.5 pg/mL-800 pg/mL，适用于体外科研实验中探索FLOT1在疾病模型、信号通路调控或药物干预下的表达变化，例如阿尔茨海默病中淀粉样蛋白代谢研究、癌症转移机制分析或靶向治疗药物的体外效果评估。可为细胞生物学、分子病理学及转化医学研究提供可靠的数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：FLOT 1 ELISA Kit; FLOT1 ELISA Kit; FLOT1_HUMAN ELISA Kit; Flotillin-1 ELISA Kit; Flotillin1 ELISA Kit; Integral membrane component of caveolae ELISA Kit; Reggie 2 ELISA Kit
缩写：FLOT1
Uniprot No.：O75955
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：12.5 pg/mL-800 pg/mL
灵敏度：3.12 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human FLOT1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 80-91 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 92-102 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 88-100 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 86-99 | |||||
回收率：
| The recovery of human FLOT1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 89-100 | ||||
| EDTA plasma (n=4) | 95 | 90-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected8002.3862.2722.3292.2084001.8681.7961.8321.7112001.3331.2841.3091.1881000.9450.9120.9290.808500.6000.5590.5800.459250.3770.3690.3730.25212.50.2560.2470.2520.13100.1240.1180.121 | ||||||
货期：3-5 working days
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