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产品描述：人细丝蛋白A(FLNA)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的FLNA含量。FLNA（细丝蛋白A）是一种细胞骨架蛋白，广泛表达于多种细胞中，参与细胞骨架的构建、信号转导和细胞运动等生物学过程。研究表明，FLNA在肿瘤的发生发展中扮演重要角色，其表达水平与肿瘤的侵袭、转移和预后密切相关。FLNA通过调节Ras/MAPK/ERK级联反应，降低MMP-9的表达，从而抑制肿瘤细胞的侵袭力。试剂盒检测范围为18.75 pg/mL-1200 pg/mL，该产品适用于科研领域对FLNA表达水平的分析，例如探究其在细胞黏附、肿瘤微环境重塑或血管生成等机制中的功能，为体外培养细胞模型、动物组织样本或生物液体中的蛋白定量研究提供高效解决方案。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ABP 280 ELISA Kit; ABP-280 ELISA Kit; Actin-binding protein 280 ELISA Kit; Alpha filamin ELISA Kit; Alpha-filamin ELISA Kit; APBX ELISA Kit; CSBS ELISA Kit; CVD1 ELISA Kit; Endothelial actin binding protein ELISA Kit; Endothelial actin-binding protein ELISA Kit; Filamin 1 ELISA Kit; Filamin A alpha ELISA Kit; Filamin A ELISA Kit; Filamin-1 ELISA Kit; Filamin-A ELISA Kit; FLN ELISA Kit; FLN-A ELISA Kit; FLN1 ELISA Kit; FLNA ELISA Kit; FLNA_HUMAN ELISA Kit; FMD ELISA Kit; MNS ELISA Kit; NHBP ELISA Kit; Non muscle filamin ELISA Kit; Non-muscle filamin ELISA Kit; OPD ELISA Kit; OPD1 ELISA Kit; OPD2 ELISA Kit; XLVD ELISA Kit; XMVD ELISA Kit
缩写：FLNA
Uniprot No.：P21333
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：18.75 pg/mL-1200 pg/mL
灵敏度：4.68 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human FLNA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 95 | ||||
| Range % | 91-104 | |||||
| 1:10 | Average % | 89 | ||||
| Range % | 87-93 | |||||
| 1:20 | Average % | 98 | ||||
| Range % | 93-102 | |||||
| 1:40 | Average % | 90 | ||||
| Range % | 87-94 | |||||
回收率：
| The recovery of human FLNA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 92-101 | ||||
| EDTA plasma (n=4) | 98 | 90-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected12002.5922.4922.5422.4016002.0121.9121.9621.8213001.3831.2831.3331.1921500.7290.7190.7240.583750.4360.4260.4310.29037.50.3440.3340.3390.19818.750.2280.2180.2230.08200.1420.1400.141 | ||||||
货期：3-5 working days
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