| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | HOX4H |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human HOXD12 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于HOXD12抗体的参考文献示例(内容基于学术文献常见结构虚构，仅供参考)：
1. **"HOXD12 Expression in Colorectal Cancer: A Prognostic Marker Study"**
*作者：Chen L, Wang Y*
摘要：通过免疫组化(使用HOXD12特异性抗体)分析结直肠癌组织样本，发现HOXD12高表达与患者生存率下降显著相关，提示其作为潜在预后标志物的可能性。
2. **"HOXD12 Regulates Limb Development via Epigenetic Modulation"**
*作者：Smith JR, Tanaka K*
摘要：研究利用HOXD12抗体进行染色质免疫沉淀(ChIP-seq)，揭示其在胚胎肢体发育中通过调控靶基因(如FGF家族)的表观遗传修饰发挥作用。
3. **"HOXD12 Antibody Validation for Western Blot and Immunofluorescence"**
*作者：Gupta A, et al.*
摘要：该文献系统验证了HOXD12抗体的特异性，确认其在多种细胞系Western blot中的单一条带及免疫荧光实验中的核定位，为后续功能研究提供工具支持。
4. **"HOXD13 and HOXD12 Functional Redundancy in Uterine Development"**
*作者：Park S, et al.*
摘要：通过敲除小鼠模型结合HOXD12抗体检测，发现HOXD12与HOXD13在子宫形态发生中存在部分功能重叠，但其缺失导致特定上皮分化异常。
The HOXD12 antibody is a tool used to detect the HOXD12 protein, a member of the HOX gene family, which plays critical roles in embryonic development, body patterning, and cellular differentiation. HOXD12. part of the HOXD cluster on chromosome 2. encodes a transcription factor that regulates target genes involved in limb development, organogenesis, and tissue homeostasis. Dysregulation of HOXD12 has been linked to developmental disorders and cancers, including colorectal, breast, and prostate malignancies, where it may act as an oncogene or tumor suppressor depending on context.
HOXD12 antibodies are typically produced in hosts like rabbits or mice using immunogenic peptides or recombinant protein fragments. These antibodies enable researchers to study HOXD12's expression patterns, subcellular localization (primarily nuclear), and interactions via techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). Validated antibodies are essential for specificity, often confirmed through knockout controls or siRNA silencing.
In research, HOXD12 antibodies help explore developmental pathways, cancer mechanisms, and potential therapeutic targets. Their application extends to diagnostic studies, particularly in cancers with HOXD12 misexpression. Proper validation ensures reliability in distinguishing HOXD12 from paralogs (e.g., HOXD11/D13), minimizing cross-reactivity. Overall, HOXD12 antibodies are vital for unraveling the gene's multifaceted roles in health and disease.
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