| 纯度 | > 85 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | ASMT |
| Uniprot No | P46597-2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-298aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSSEDQAYR LLNDYANGFM VSQVLFAACE LGVFDLLAEA PGPLDVAAVA AGVRASAHGT ELLLDICVSL KLLKVETRGG KAFYRNTELS SDYLTTVSPT SQCSMLKYMG RTSYRCWGHL ADAVREGRNQ YLETFGVPAE ELFTAIYRSE GERLQFMQAL QEVWSVNGRS VLTAFDLSVF PLMCDLGGDF FKDPLPEADL YILARVLHDW ADGKCSHLLE RIYHTCKPGG GILVIESLLD EDRRGPLLTQ LYSLNMLVQT EGQERTPTHY HMLLSSAGFR DFQFKKTGAI YDAILARK |
| 预测分子量 | 35 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ASMT(N-乙酰血清素甲基转移酶)重组蛋白研究的模拟参考文献示例(仅供参考，实际文献需通过学术数据库查询)：
1. **《Expression and Purification of Recombinant Human ASMT in Escherichia coli》**
- 作者：Zhang L, et al.
- 摘要：研究报道了人源ASMT基因在大肠杆菌中的重组表达及纯化方法，优化了诱导条件并通过亲和层析获得高纯度蛋白，酶活实验证实重组蛋白具有催化合成褪黑激素的功能。
2. **《Structural Insights into ASMT through Crystallography of Recombinant Protein》**
- 作者：Kim S, et al.
- 摘要：利用昆虫细胞表达系统制备重组ASMT蛋白，通过X射线晶体学解析其三维结构，揭示了底物结合的关键位点及催化机制，为褪黑激素合成相关疾病的研究提供结构基础。
3. **《Functional Characterization of Recombinant ASMT Variants in Psychiatric Disorders》**
- 作者：Marquez-Bravo B, et al.
- 摘要：通过构建ASMT重组蛋白的突变体，分析其酶活性与自闭症、抑郁症患者中基因突变的关联，发现部分突变导致酶活显著下降，提示ASMT功能异常与精神疾病的相关性。
4. **《Optimization of ASMT Recombinant Production in Pichia pastoris for Biomedical Applications》**
- 作者：Wang Y, et al.
- 摘要：探讨在毕赤酵母中高效表达重组ASMT的发酵条件，开发了高产量纯化工艺，并验证其在体外模型中的生物活性，为大规模生产褪黑激素前体提供技术方案。
**注意**：以上文献信息为模拟示例，具体研究请通过PubMed、Web of Science或Google Scholar检索关键词(如“ASMT recombinant protein”“melatonin synthase expression”)获取真实文献。
**Background of ASMT Recombinant Proteins**
Acetylserotonin O-methyltransferase (ASMT), also known as hydroxyindole-O-methyltransferase (HIOMT), is a key enzyme in the melatonin biosynthesis pathway. It catalyzes the final step of melatonin production by converting N-acetylserotonin to melatonin via methylation. Melatonin, a neurohormone primarily synthesized in the pineal gland, regulates circadian rhythms, sleep-wake cycles, and antioxidant activity. Dysregulation of ASMT expression or function has been linked to sleep disorders, mood disorders (e.g., depression), and neurodevelopmental conditions such as autism spectrum disorder (ASD).
Recombinant ASMT proteins are engineered using genetic cloning techniques, where the ASMT gene is inserted into expression systems (e.g., *E. coli*, yeast, or mammalian cells*) to produce purified, functional enzymes. These recombinant proteins retain the catalytic activity of native ASMT, enabling researchers to study its structure, kinetics, and interactions in vitro. They are critical tools for investigating melatonin-related pathologies, screening potential therapeutics, and understanding genetic mutations that impair enzyme activity.
Recent studies highlight ASMT's role beyond melatonin synthesis, including its potential involvement in mitochondrial function and immune modulation. However, challenges remain in optimizing recombinant ASMT stability and solubility for structural studies. Advances in protein engineering, such as codon optimization and fusion tags, have improved yield and functionality.
Overall, ASMT recombinant proteins are vital for both basic research and translational applications, offering insights into circadian biology and paving the way for therapeutic strategies targeting melatonin pathway disorders.
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