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产品描述：人蛋白FAM132A(FAM132A)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆、细胞裂解物样本中的FAM132A含量。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，该产品可为研究者分析FAM132A在肥胖、糖尿病相关模型中的表达动态，探索其在细胞能量应激响应机制中的作用提供可靠工具，同时支持肿瘤微环境、神经退行性疾病等领域的蛋白功能研究本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：C1QTNF12 ELISA Kit; C1QDC2 ELISA Kit; CTRP12 ELISA Kit; FAM132AAdipolin ELISA Kit; Adipose-derived insulin-sensitizing factor ELISA Kit; C1q and TNF related protein 12 ELISA Kit; Complement C1q tumor necrosis factor-related protein 12) [Cleaved into: Adipolin fC1QTNF12 ELISA Kit; Adipolin fCTRP12 ELISA Kit; Adipolin full-length form); Adipolin gC1QTNF12 ELISA Kit; Adipolin cleaved form ELISA Kit; Adipolin gCTRP12)] ELISA Kit
缩写：FAM132A
Uniprot No.：Q5T7M4
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates, cell lysates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human FAM132A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 85-89 | |||||
| 1:2 | Average % | 99 | ||||
| Range % | 91-102 | |||||
| 1:4 | Average % | 100 | ||||
| Range % | 93-108 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 90-99 | |||||
回收率：
| The recovery of human FAM132A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 87-96 | ||||
| EDTA plasma (n=4) | 94 | 90-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.3802.3562.3682.23710001.8151.7761.7961.6655001.2201.1981.2091.0782500.6690.6660.6680.5371250.4380.4240.4310.30062.50.2260.2220.2240.09331.250.1810.1780.1800.04900.1340.1280.131 | ||||||
货期：3-5 working days
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