| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/10000 | Human,Mouse,Rat |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human COX17 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于COX17抗体的3篇参考文献及其摘要概括：
1. **文献名称**：*"Cloning and characterization of COX17. a yeast gene involved in copper metabolism and mitochondrial function"*
**作者**：Glerum DM, Shtanko A, Tzagoloff A
**摘要**：该研究克隆了酵母COX17基因，发现其编码的蛋白参与线粒体内铜离子的转运，对细胞色素c氧化酶的组装至关重要。通过抗体检测证实COX17定位于线粒体，并调控铜的递送过程。
2. **文献名称**：*"Human COX17 is a copper-dependent chaperone for cytochrome c oxidase"*
**作者**：Horng YC, Cobine PA, Maxfield AB, et al.
**摘要**：研究利用COX17抗体分析人源蛋白功能，发现COX17通过结合铜离子协助其转运至线粒体，并与SCO1、COX11等分子协同作用，确保细胞色素c氧化酶的活性中心形成。
3. **文献名称**：*"Regulation of COX17 expression during hypoxia and copper deprivation"*
**作者**：Vladimirski MM, Barrientos A
**摘要**：通过免疫印迹和免疫荧光技术(使用COX17特异性抗体)，发现低氧和铜缺乏条件下，COX17表达上调，提示其在维持线粒体氧化磷酸化中的动态调控作用。
4. **文献名称**：*"Copper-dependent interaction between COX17 and SCO1 promotes cellular copper homeostasis"*
**作者**：Leary SC, Cobine PA, Kaufman BA, et al.
**摘要**：研究利用COX17抗体进行共免疫沉淀实验，揭示了COX17与SCO1蛋白的铜依赖性相互作用，这对维持细胞内铜稳态及线粒体呼吸链功能至关重要。
以上文献均通过COX17抗体探究了该蛋白在铜代谢、线粒体功能及疾病中的分子机制。
COX17 is a key protein involved in the assembly of cytochrome c oxidase (COX), the terminal enzyme in the mitochondrial electron transport chain responsible for oxidative phosphorylation. As a copper chaperone, COX17 facilitates the delivery of copper ions to the COX complex, which is essential for its catalytic activity. Located primarily in the mitochondrial intermembrane space, COX17 interacts with other assembly factors (e.g., SCO1. SCO2) to ensure proper metallation of COX subunits. Dysregulation of COX17 has been linked to mitochondrial disorders, neurodegenerative diseases (e.g., Alzheimer’s, Parkinson’s), and cancer, reflecting its critical role in cellular energy production and redox homeostasis.
COX17 antibodies are vital tools for studying its expression, localization, and function. They are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to quantify COX17 levels in tissues or cells, assess its subcellular distribution, and investigate its involvement in disease mechanisms. Commercially available antibodies are typically raised against specific epitopes of human or murine COX17. with validations including knockout controls to confirm specificity. Researchers also employ these antibodies to explore COX17’s interplay with other copper-trafficking proteins or its response to metabolic stressors, such as hypoxia or copper deficiency, providing insights into mitochondrial health and therapeutic targeting.
×
