首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人细胞绒毛蛋白/埃兹蛋白(cytovillin/ezrin)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的EZR含量。EZR（Ezrin）是ERM蛋白家族的重要成员，主要功能是连接细胞骨架与质膜，参与细胞形态维持、细胞粘附、细胞迁移和细胞信号传导等生理过程。EZR在多种肿瘤的发生、发展和转移中发挥重要作用，其异常表达与肿瘤细胞的侵袭、迁移和化疗耐药性相关。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于基础科研领域研究细胞骨架动态变化、上皮 - 间质转化机制、细胞极性建立以及相关疾病模型中埃兹蛋白的表达调控，例如肿瘤微环境中的侵袭性研究、免疫细胞趋化运动分析或肠道上皮屏障功能探索。通过宽泛的样本兼容性，研究者可同步分析生物体液、组织及体外培养细胞中的蛋白水平，为跨物种或疾病模型的分子机制研究提供标准化工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Villin 2 ezrin ELISA Kit; CVIL ELISA Kit; CVL ELISA Kit; Cytovillin 2 ELISA Kit; Cytovillin ELISA Kit; DKFZp762H157 ELISA Kit; Epididymis secretory protein Li 105 ELISA Kit; EZR ELISA Kit; EZRI_HUMAN ELISA Kit; Ezrin ELISA Kit; FLJ26216 ELISA Kit; HEL S 105 ELISA Kit; MGC1584 ELISA Kit; p81 ELISA Kit; VIL 2 ELISA Kit; VIL2 ELISA Kit; Villin 2 (ezrin) ELISA Kit; Villin 2 ELISA Kit; Villin-2 ELISA Kit; Villin2 ELISA Kit
缩写：EZR
Uniprot No.：P15311
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human cytovillin/ezrin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 91-101 | |||||
| 1:2 | Average % | 100 | ||||
| Range % | 95-104 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 87-97 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 93-103 | |||||
回收率：
| The recovery of human cytovillin/ezrin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 88-95 | ||||
| EDTA plasma (n=4) | 95 | 89-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected201.7101.7541.7321.603101.1831.1671.1751.04650.7490.7550.7520.6232.50.3770.3660.3720.2431.250.2920.3080.3000.1710.6250.2220.2370.2300.1010.3120.1750.1660.1710.04200.1280.1300.129 | ||||||
货期：3-5 working days
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