首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人上皮调节蛋白(EREG)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的EREG含量。EREG即表皮调节素，是表皮生长因子家族成员。它可与EGFR结合激活下游信号通路，在细胞的增殖、分化及存活等方面起作用。研究发现其在多种肿瘤中异常表达，与肿瘤的发生、发展、侵袭转移等相关，是潜在的肿瘤治疗靶点。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于体外培养细胞分泌研究、疾病动物模型体液检测、肿瘤微环境分析等科研场景，为探究EREG在癌症生物学、炎症调控及再生医学中的功能机制提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Epiregulin ELISA Kit; EPR ELISA Kit; ER ELISA Kit; Ereg ELISA Kit; EREG_HUMAN ELISA Kit; Proepiregulin ELISA Kit
缩写：EREG
Uniprot No.：O14944
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human EREG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 84-89 | |||||
| 1:2 | Average % | 99 | ||||
| Range % | 94-103 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 90-95 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 89-98 | |||||
回收率：
| The recovery of human EREG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 87-99 | ||||
| EDTA plasma (n=4) | 89 | 85-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.1001.9522.0261.9365001.3511.3421.3471.2572500.7880.7790.7840.6941250.4780.4680.4730.38362.50.2900.2820.2860.19631.20.2190.2040.2120.12215.60.1560.1510.1540.06400.0920.0880.090 | ||||||
货期：3-5 working days
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