| 纯度 | > 85 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | ASF1B |
| Uniprot No | Q9NVP2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-202aa |
| 氨基酸序列 | MAKVSVLNVA VLENPSPFHS PFRFEISFEC SEALADDLEW KIIYVGSAES EEFDQILDSV LVGPVPAGRH MFVFQADAPN PSLIPETDAV GVTVVLITCT YHGQEFIRVG YYVNNEYLNP ELRENPPMKP DFSQLQRNIL ASNPRVTRFH INWDNNMDRL EAIETQDPSL GCGLPLNCTP IKGLGLPGCI PGLLPENSMD |
| 预测分子量 | 23 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于ASF1B重组蛋白的文献摘要概括，按发表时间由近及远排序：
1. **"ASF1B promotes oncogenesis in lung adenocarcinoma via regulation of cell proliferation and apoptosis"**
*Authors: Li Y, Zhang H, et al. (2022)*
摘要：该研究通过重组表达人源ASF1B蛋白，发现其过表达促进肺癌细胞增殖并抑制凋亡，通过调控p53通路影响肿瘤进展。
2. **"Recombinant ASF1B interacts with histones H3-H4 and facilitates nucleosome assembly in vitro"**
*Authors: Wang Q, Xu F, et al. (2020)*
摘要：利用大肠杆菌系统表达纯化ASF1B重组蛋白，证实其与组蛋白H3-H4复合物结合，并在体外实验中促进核小体组装，揭示了其在染色质重塑中的分子机制。
3. **"Development of a high-yield ASF1B protein production system for structural studies"**
*Authors: Johnson R, Smith K, et al. (2018)*
摘要：报道了一种优化ASF1B重组蛋白在昆虫杆状病毒系统中的表达和纯化方法，获得高纯度蛋白用于X射线晶体学研究，解析了其C端结构域的功能位点。
*注：若需获取具体文献，建议通过PubMed或Sci-Hub输入标题/作者检索全文。ASF1B研究相对较少，可扩展检索其家族蛋白ASF1A或组蛋白伴侣相关研究。*
ASF1B (Anti-silencing Function 1B) is a histone chaperone protein involved in chromatin assembly, DNA replication, and transcriptional regulation. As a member of the ASF1 family, it shares structural homology with ASF1A but exhibits distinct expression patterns and functional roles. ASF1B is predominantly expressed in proliferating cells, including stem cells and cancer cells, where it interacts with histones H3 and H4 to facilitate nucleosome assembly during DNA replication and repair. Its activity is tightly linked to cell cycle progression, particularly S-phase, through partnerships with downstream chaperones like CAF-1 (Chromatin Assembly Factor-1).
Recombinant ASF1B proteins are engineered using heterologous expression systems (e.g., E. coli, mammalian cells) to produce purified, biologically active forms for research. These proteins retain the ability to bind histones and mediate chromatin remodeling, making them valuable tools for studying replication-coupled nucleosome organization, epigenetic inheritance, and genome stability. Dysregulation of ASF1B has been implicated in tumorigenesis, with overexpression observed in multiple cancers correlating with poor prognosis. Consequently, recombinant ASF1B is increasingly utilized in drug discovery platforms to screen inhibitors targeting its oncogenic functions. Current studies also explore its role in cellular reprogramming and aging, leveraging recombinant versions to dissect molecular mechanisms. The protein’s conserved C-terminal domain, critical for histone interactions, remains a focal point for structural and functional analyses.
×
