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产品描述：人环氧化物水解酶2(EPHX2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的EPHX2含量。EPHX2是一个值得关注的靶点。该基因编码环氧化物水解酶，参与体内多种环氧化物代谢。研究机制方面，聚焦其在药物代谢及疾病发生发展中的作用，如在癌症、心血管疾病等方面，探究其功能机制或可为防治提供新思路。试剂盒检测范围为23.44 pg/mL-1500 pg/mL，其应用场景包括基础科研中探究EPHX2在代谢调控、炎症信号通路中的作用机制，或药理学研究中评估化合物对EPHX2表达水平的影响，为心血管疾病、糖尿病等领域的体外实验研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Bifunctional epoxide hydrolase 2 ELISA Kit; CEH ELISA Kit; Cytosolic epoxide hydrolase ELISA Kit; EPHX2 ELISA Kit; Epoxide hydratase ELISA Kit; Epoxide hydrolase 2 ELISA Kit; Epoxide hydrolase 2 cytoplasmic ELISA Kit; epoxide hydrolase 2, cytosolic ELISA Kit; Epoxide hydrolase soluble ELISA Kit; HYES_HUMAN ELISA Kit; SEH ELISA Kit; Soluble epoxide hydrolase ELISA Kit
缩写：EPHX2
Uniprot No.：P34913
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：23.44 pg/mL-1500 pg/mL
灵敏度：5.86 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human EPHX2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 89 | |||||
| Range % | 84-93 | ||||||
| 1:2 | Average % | 97 | |||||
| Range % | 92-101 | ||||||
| 1:4 | Average % | 91 | |||||
| Range % | 85-95 | ||||||
| 1:8 | Average % | 93 | |||||
| Range % | 89-97 | ||||||
回收率：
| The recovery of human EPHX2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 103 | 99-108 | |||||
| EDTA plasma (n=4) | 93 | 89-97 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected15002.2092.3262.2682.1137501.7201.6641.6921.5373751.1941.2131.2041.049187.50.8710.8570.8640.70993.750.5460.5320.5390.38446.880.3830.4010.3920.23723.440.2570.2490.2530.09800.1520.1580.155 | |||||||
货期：3-5 working days
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