首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人B型肝配蛋白受体2(EPHB2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的EPHB2含量。EPHB2 为受体酪氨酸激酶 Eph 家族成员，在胚胎发育、血管生成等过程起重要作用。研究机制上，其与配体 ephrin-B 结合后，可激活下游信号通路，调控细胞黏附、迁移等，异常表达与肿瘤等疾病发生发展相关。试剂盒检测范围为0.625 ng/mL-40 ng/mL，适用于基础研究中对EPHB2表达水平的动态监测，如肿瘤生物学、神经退行性疾病模型或组织再生机制等领域的体外实验，为探索EPHB2在细胞间通讯、病理生理过程中的功能提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：cek5 ELISA Kit; Developmentally regulated EPH related tyrosine kinase ELISA Kit; DRT ELISA Kit; EK5 ELISA Kit; ELK related protein tyrosine kinase ELISA Kit; Eph receptor B2 ELISA Kit; EPH tyrosine kinase 3 ELISA Kit; EPH-like kinase 5 ELISA Kit; EPHB2 ELISA Kit; EPHB2_HUMAN ELISA Kit; Ephrin type B receptor 2 ELISA Kit; Ephrin type-B receptor 2 ELISA Kit; EPHT 3 ELISA Kit; ERK ELISA Kit; ETECK ELISA Kit; hEK5 ELISA Kit; Nuk ELISA Kit; Prkm 5 ELISA Kit; Receptor protein tyrosine kinase HEK 5 ELISA Kit; Renal carcinoma antigen NY-REN-47 ELISA Kit; Sek 3 ELISA Kit; Tyro 5 ELISA Kit; Tyrosine protein kinase receptor CEK 5 ELISA Kit; Tyrosine protein kinase receptor EPH 3 ELISA Kit; Tyrosine protein kinase receptor QEK 5 ELISA Kit; Tyrosine-protein kinase receptor EPH-3 ELISA Kit; Tyrosine-protein kinase TYRO5 ELISA Kit
缩写：EPHB2
Uniprot No.：P29323
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human EPHB2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 91 | |||||
| Range % | 88-96 | ||||||
| 1:2 | Average % | 96 | |||||
| Range % | 90-101 | ||||||
| 1:4 | Average % | 97 | |||||
| Range % | 90-104 | ||||||
| 1:8 | Average % | 101 | |||||
| Range % | 96-106 | ||||||
回收率：
| The recovery of human EPHB2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 91 | 88-95 | |||||
| EDTA plasma (n=4) | 96 | 92-100 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected402.6072.6882.6482.462201.9351.9851.9601.774101.1251.2121.1690.98350.6830.6980.6910.5052.50.4420.4470.4450.2591.250.3090.3230.3160.1300.6250.2300.2460.2380.05200.1820.1900.186 | |||||||
货期：3-5 working days
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