首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠脑啡肽(ENK)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的ENK含量。试剂盒检测范围为0.625 ng/mL-40 ng/mL，该产品适用于神经科学、药理学等领域的基础研究，例如探究疼痛机制模型、应激反应调控或药物干预对ENK表达的影响，为神经系统功能研究提供可靠的实验工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：ENK
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat ENK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 106 | |||||
| Range % | 103-111 | ||||||
| 1:2 | Average % | 95 | |||||
| Range % | 92-98 | ||||||
| 1:4 | Average % | 90 | |||||
| Range % | 86-95 | ||||||
| 1:8 | Average % | 93 | |||||
| Range % | 90-96 | ||||||
回收率：
| The recovery of rat ENK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 103 | 99-106 | |||||
| EDTA plasma (n=4) | 91 | 85-95 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected 402.3472.4282.3882.291 201.6511.7401.6961.599 101.3441.2391.2921.195 50.8370.9000.8690.772 2.50.4850.4770.4810.384 1.250.3090.2890.2990.202 0.6250.2000.2050.2030.106 00.0950.0980.097 | |||||||
货期：3-5 working days
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