首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人锯齿状同源异型盒蛋白2(EN2)酶联免疫试剂盒 双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的EN2含量。试剂盒检测范围为0.312 ng/mL-20 ng/mL，该产品适用于体外研究EN2蛋白在疾病模型中的表达变化，如肿瘤生物学机制探索、基因功能研究或药物干预效果评估等领域，为科研人员提供稳定可靠的分析工具，助力于癌症相关分子标记物筛选及分子通路研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：EN2 ELISA Kit; Homeobox protein engrailed-2 ELISA Kit; Homeobox protein en-2 ELISA Kit; Hu-En-2 ELISA Kit
缩写：EN2
Uniprot No.：P19622
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human EN2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 89-97 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 86-96 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 96-105 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 89-98 | |||||
回收率：
| The recovery of human EN2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 83 | 80-87 | ||||
| EDTA plasma (n=4) | 92 | 86-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.4662.3652.4162.271101.7591.6581.7091.56451.0281.0071.0180.8732.50.6150.6040.6100.4651.250.3910.4020.3970.2520.6250.2580.2680.2630.1180.3120.2210.2240.2230.07800.1440.1450.145 | ||||||
货期：3-5 working days
×
