首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人Adropin(ENHO)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ENHO含量。Adropin（ENHO）是一种调节能量稳态的分泌蛋白，由Enho基因编码。研究表明，Adropin在哺乳动物中参与摄食行为调控，并可能具有广泛的生理作用。其研究机制涉及能量平衡、葡萄糖和脂肪酸代谢调控，与肥胖、糖尿病等代谢性疾病相关。试剂盒检测范围为1.56 pg/mL-100 pg/mL，适用于基础研究中Adropin在代谢调控机制探索、疾病模型动态变化分析或药物干预效果评估等场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Adropin ELISA Kit; C9orf165 ELISA Kit; Energy homeostasis associated protein ELISA Kit; Energy homeostasis-associated protein ELISA Kit; ENHO ELISA Kit; ENHO_HUMAN ELISA Kit; PRO830 ELISA Kit; UNQ470 ELISA Kit
缩写：ENHO
Uniprot No.：Q6UWT2
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 pg/mL-100 pg/mL
灵敏度：0.39 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ENHO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 94 | ||||
| Range % | 88-99 | |||||
| 1:200 | Average % | 90 | ||||
| Range % | 84-95 | |||||
| 1:400 | Average % | 98 | ||||
| Range % | 91-99 | |||||
| 1:800 | Average % | 95 | ||||
| Range % | 90-100 | |||||
回收率：
| The recovery of human ENHO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 87 | 80-94 | ||||
| EDTA plasma (n=4) | 100 | 94-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected1002.0742.0992.0871.923501.7981.7651.7821.618251.3971.3841.3911.22712.50.9820.9430.9630.7996.250.5830.5960.5900.4263.120.3710.3910.3810.2171.560.2960.2810.2890.12500.1620.1660.164 | ||||||
货期：3-5 working days
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