Rat Epidermal growth factor,EGF ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-E08029r

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
大鼠表皮生长因子(EGF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的EGF含量。EGF 即表皮生长因子，是一种广泛存在于人体多种组织中的小分子多肽。它能与表皮生长因子受体结合，激活下游信号通路，参与细胞的增殖、分化和迁移等过程。目前在肿瘤、皮肤修复等领域研究较多，其研究有助于揭示疾病机制及开发新疗法。试剂盒检测范围为3.12 pg/mL-200 pg/mL，适用于大鼠疾病模型研究、药物干预效果评估或基础科研中EGF动态变化的定量分析，支持科研人员高效开展与皮肤损伤修复、肿瘤微环境或炎症调控相关的分子机制研究，为探索EGF在大鼠生理及病理过程中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Egf ELISA Kit; Pro-epidermal growth factor ELISA Kit; EGF) [Cleaved into: Epidermal growth factor] ELISA Kit
缩写：
EGF
Uniprot No.：
P07522
种属：
Rattus norvegicus (Rat)
样本类型：
serum, plasma, tissue homogenates
检测范围：
3.12 pg/mL-200 pg/mL
灵敏度：
0.78 pg/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Others
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat EGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:100	Average %	91
	Range %	86-98
1:200	Average %	96
	Range %	92-104
1:400	Average %	85
	Range %	81-88
1:800	Average %	88
	Range %	83-94

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
200	2.503	2.478	2.491	2.347
100	2.156	2.168	2.162	2.018
50	1.564	1.560	1.562	1.418
25	1.024	1.001	1.013	0.869
12.5	0.586	0.549	0.568	0.424
6.25	0.364	0.345	0.355	0.211
3.12	0.258	0.264	0.261	0.117
0	0.149	0.138	0.144