首页 / 产品 / ELISA试剂盒 / 兔ELISA试剂盒
产品描述：兔子表皮生长因子(EGF)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的EGF含量。表皮生长因子（EGF）是一种重要的生长因子，属于表皮生长因子家族，通过与其受体EGFR结合，激活细胞内的信号传导途径，促进细胞增殖、分化、迁移和存活。EGF在胚胎发育、组织再生和伤口愈合中发挥关键作用，同时其异常表达与多种疾病，尤其是癌症的发生发展密切相关。试剂盒检测范围为0.5 ng/mL-8 ng/mL，特别适用于探究EGF在皮肤屏障功能、消化道黏膜修复、创伤愈合等研究领域的调控机制。科研人员可利用分析不同生理/病理条件下体液和组织中的EGF动态变化，为研究生长因子在再生医学、肿瘤微环境中的作用提供量化依据，其宽检测范围设计可有效应对复杂生物样本中目标蛋白浓度的显著波动。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：EGF
Uniprot No.：G1TBJ6
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma, tissue homogenates
检测范围：0.5 ng/mL-8 ng/mL
灵敏度：0.25 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit EGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 111 | ||||
| Range % | 100-115 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 84-97 | |||||
| 1:4 | Average % | 100 | ||||
| Range % | 90-104 | |||||
| 1:8 | Average % | 100 | ||||
| Range % | 93-104 | |||||
回收率：
| The recovery of rabbit EGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-98 | ||||
| EDTA plasma (n=4) | 92 | 87-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average80.1470.1410.14440.3110.3220.31720.5010.5340.51810.9260.9020.9140.51.9321.9661.949 | ||||||
货期：3-5 working days
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