| 纯度 | > 90 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | ASCC1 |
| Uniprot No | Q8N9N2-2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-357aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMEVLRPQ LIRIDGRNYR KNPVQEQTYQ HEEDEEDFYQ GSMECADEPC DAYEVEQTPQ GFRSTLRAPS LLYKHIVGKR GDTRKKIEME TKTSISIPKP GQDGEIVITG QHRNGVISAR TRIDVLLDTF RRKQPFTHFL AFFLNEVEVQ EGFLRFQEEV LAKCSMDHGV DSSIFQNPKK LHLTIGMLVL LSEEEIQQTC EMLQQCKEEF INDISGGKPL EVEMAGIEYM NDDPGMVDVL YAKVHMKDGS NRLQELVDRV LERFQASGLI VKEWNSVKLH ATVMNTLFRK DPNAEGRYNL YTAEGKYIFK ERESFDGRNI LKLFENFYFG SLKLNSIHIS QRFTVDSFGN YASCGQIDFS |
| 预测分子量 | 44 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ASCC1重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：Structural and functional analysis of the ASCC1-ALKBH3 complex in DNA repair
**作者**：Smith J, et al.
**摘要**：本研究解析了ASCC1重组蛋白与ALKBH3形成的复合物结构，揭示其通过碱基切除修复途径参与DNA损伤修复的分子机制，并利用重组蛋白验证了二者在体外修复氧化损伤DNA的功能。
2. **文献名称**：ASCC1 regulates NF-κB signaling via interaction with IKKγ
**作者**：Li X, et al.
**摘要**：通过表达纯化人源ASCC1重组蛋白，发现其与IKKγ直接互作，调控NF-κB信号通路激活。实验表明ASCC1缺失会抑制炎症因子诱导的NF-κB核转位。
3. **文献名称**：Recombinant ASCC1 enhances transcriptional coactivation through histone modification
**作者**：Park S, et al.
**摘要**：研究证实ASCC1重组蛋白能与组蛋白乙酰转移酶p300协同作用，通过改变染色质修饰状态增强核受体介导的基因转录，为ASCC1在表观遗传调控中的功能提供证据。
注：上述文献信息为示例性概括，实际文献需通过学术数据库检索确认。若研究领域较新，可关注ASCC1在DNA修复、转录共激活复合物或癌症关联中的近期研究。
The ASCC1 (Activating Signal Cointegrator Complex Subunit 1) recombinant protein is a key component of the ASCC complex, a multiprotein assembly involved in transcriptional regulation and DNA damage response. ASCC1. along with ASCC2 and ASCC3. forms part of this evolutionarily conserved complex that interacts with nuclear receptors and transcription factors to modulate gene expression. Structurally, ASCC1 contains tetratricopeptide repeat (TPR) domains, which mediate protein-protein interactions critical for its functional integration into the complex.
Biologically, ASCC1 plays dual roles. It contributes to transcriptional coactivation by facilitating chromatin remodeling and recruiting RNA polymerase II. Additionally, it participates in alkylation damage repair through its association with ALKBH3. a dioxygenase that repairs methylated DNA lesions. This repair mechanism is vital for maintaining genomic stability, particularly in response to environmental alkylating agents or chemotherapeutic drugs.
Recombinant ASCC1 protein is typically produced using bacterial or mammalian expression systems, often fused with tags like His or GST for purification and detection. Its study enables detailed biochemical characterization, including interaction mapping with DNA repair enzymes or transcriptional partners. Researchers utilize this protein to investigate mechanisms underlying transcription-coupled repair, cancer progression (where ASCC1 dysregulation is observed in certain tumors), and cellular responses to DNA-damaging therapies. Current research also explores its potential as a biomarker or therapeutic target in malignancies linked to defective DNA repair pathways.
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