| 纯度 | > 90 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | ASB8 |
| Uniprot No | Q9H765 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-288aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMSSSMWYIMQSIQSKYSLSERLIRTIA AIRSFPHDNVEDLIRGGADVNCTHGTLKPLHCACMVSDADCVELLLEKGA EVNALDGYNRTALHYAAEKDEACVEVLLEYGANPNALDGNRDTPLHWAAF KNNAECVRALLESGASVNALDYNNDTPLSWAAMKGNLESVSILLDYGAEV RVINLIGQTPISRLVALLVRGLGTEKEDSCFELLHRAVGHFELRKNGTMP REVARDPQLCEKLTVLCSAPGTLKTLARYAVRRSLGLQYLPDAVKGLPLP ASLKEYLLLLE |
| 预测分子量 | 34 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ASB8重组蛋白的3篇模拟参考文献及其摘要概括(注：文献信息为虚构示例，仅供格式参考)：
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1. **文献名称**: *Cloning and Expression of Recombinant Human ASB8 in Escherichia coli*
**作者**: Li, X. et al.
**摘要**: 研究报道了人源ASB8基因的克隆及在大肠杆菌中的可溶性表达，通过His标签亲和层析纯化获得高纯度蛋白，并验证其与E3泛素连接酶复合物的相互作用。
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2. **文献名称**: *Functional Characterization of ASB8 in TGF-β Signaling via Ubiquitination Assays*
**作者**: Wang, Y. et al.
**摘要**: 利用昆虫细胞系统表达ASB8重组蛋白，发现其通过介导TGF-β受体降解负向调控信号通路，为ASB8在肿瘤发生中的作用提供了机制证据。
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3. **文献名称**: *Structural Insights into ASB8-Elongin BC Complex by Cryo-EM*
**作者**: Chen, J. et al.
**摘要**: 通过冷冻电镜解析ASB8与Elongin B/C复合物的三维结构，揭示了其底物识别域的关键位点，为设计靶向ASB8的小分子抑制剂奠定基础。
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提示：实际文献需通过PubMed/Google Scholar检索关键词“ASB8 recombinant”或“ASB8 ubiquitin”，建议结合具体研究场景筛选近年高相关度论文。
ASB8 (Ankyrin repeat and SOCS box protein 8) is a member of the ASB family characterized by a C-terminal SOCS (suppressor of cytokine signaling) box domain and an N-terminal ankyrin repeat domain. The ankyrin repeats mediate protein-protein interactions, often involved in substrate recognition, while the SOCS box facilitates interactions with Elongin B/C, Cullin5. and Rbx2 to form an E3 ubiquitin ligase complex. This complex targets specific proteins for ubiquitination-mediated proteasomal degradation, linking ASB8 to cellular processes like signal transduction, apoptosis, and cell cycle regulation.
ASB8 is ubiquitously expressed, with higher levels in tissues such as the brain, heart, and skeletal muscle. Studies suggest its involvement in diverse pathways, including modulating TGF-β signaling by promoting the degradation of Smad proteins and regulating mitochondrial dynamics through interactions with fission/fusion machinery. Dysregulation of ASB8 has been implicated in pathological conditions, including certain cancers. For example, it may act as a tumor suppressor in breast cancer by destabilizing oncogenic proteins, while paradoxically exhibiting pro-tumor effects in colorectal cancer, highlighting context-dependent roles.
Recombinant ASB8 protein, typically produced in *E. coli* or mammalian expression systems, retains functional domains for biochemical studies. It is used to investigate substrate binding, ubiquitination assays, and structural analysis. Researchers also employ it to explore therapeutic strategies targeting E3 ligase activity or protein stability pathways. Despite progress, ASB8's full substrate repertoire and regulatory mechanisms remain understudied, warranting further exploration of its physiological and pathological significance.
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