| 纯度 | > 90 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | ARPP19 |
| Uniprot No | P56211 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-112aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMSAEVPE AASAEEQKEM EDKVTSPEKA EEAKLKARYP HLGQKPGGSD FLRKRLQKGQ KYFDSGDYNM AKAKMKNKQL PTAAPDKTEV TGDHIPTPQD LPQRKPSLVA SKLAG |
| 预测分子量 | 15 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ARPP19重组蛋白的3篇文献示例(注：以下内容为模拟概括，实际文献需根据具体数据库检索确认)：
1. **文献名称**：*ARPP19 regulates mitotic progression through phosphorylation by Greatwall kinase*
**作者**：H. H. et al.
**摘要**：研究揭示了ARPP19重组蛋白在体外被Greatwall激酶磷酸化的机制，证实其通过抑制PP2A/B55磷酸酶复合物调控有丝分裂进入和退出。
2. **文献名称**：*Recombinant ARPP19 expression and its role in oocyte maturation*
**作者**：M. L. et al.
**摘要**：报道了ARPP19重组蛋白在非洲爪蟾卵母细胞中的表达与纯化方法，证明其作为cAMP/PKA信号下游效应分子，通过调控CDK1活性参与减数分裂恢复。
3. **文献名称**：*Structural insights into ARPP19-PP2A interaction by cryo-EM*
**作者**：T. S. et al.
**摘要**：利用冷冻电镜解析重组ARPP19与PP2A/B55复合物的结构，阐明其通过保守的N端结构域结合PP2A，抑制去磷酸化活性以维持有丝分裂进程。
(如需具体文献，建议在PubMed或Google Scholar中检索关键词“ARPP19 recombinant protein”或结合研究领域筛选。)
ARPP19 (cAMP-regulated phosphoprotein 19) is a conserved regulatory protein involved in critical cellular processes, notably cell cycle progression and signal transduction. Initially identified as a substrate for cAMP-dependent protein kinase (PKA), ARPP19 plays a key role in mitosis by regulating the transition from G2 phase to mitosis (G2/M checkpoint) and mitotic exit. It functions through interactions with protein phosphatases and kinases, particularly PP2A/B55δ and the Greatwall kinase (MASTL). During mitosis, ARPP19 is phosphorylated by MASTL at a conserved site (e.g., Thr109 in humans), enabling it to inhibit PP2A/B55δ activity. This inhibition maintains CDK1-cyclin B activity, ensuring proper mitotic progression and preventing premature exit. Dysregulation of this pathway is linked to mitotic errors and genomic instability.
Recombinant ARPP19 protein is commonly produced in bacterial or mammalian expression systems for functional studies. Its recombinant form enables researchers to dissect molecular mechanisms, including phosphorylation-dependent interactions, phosphatase regulation, and its role in cell cycle checkpoints. ARPP19 has also been implicated in diseases such as cancer, where its overexpression correlates with poor prognosis, suggesting its potential as a therapeutic target. Recombinant variants (e.g., phosphomimetic or phosphorylation-deficient mutants) are used to study structure-function relationships. Additionally, ARPP19's cAMP-responsive domains and regulatory motifs make it a model for exploring cross-talk between cAMP signaling and cell cycle control. Its study contributes to understanding fundamental biology and developing strategies to target cell cycle-related disorders.
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