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Human growth-regulated oncogeneα/melanoma growth stimulating activity,GROα/MGSA ELISA Kit

  • 中文名: 人生长调节致癌基因α/黑素瘤生长刺激因子(GROα/CXCL1/MGSA)ELISA Kit
货号: IPD-E09150h
Price: ¥2500
数量:
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产品详情

  • 产品描述:
    人生长调节致癌基因α/黑素瘤生长刺激因子(GROα/CXCL1/MGSA)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的CXCL1含量。CXCL1属于趋化因子,在炎症、免疫反应等生理过程中发挥重要作用。其背景与机体防御和疾病发生相关。研究机制上,它能与特定受体结合,介导细胞趋化,在肿瘤生长、血管生成以及炎症性疾病进展中扮演关键角色,是潜在治疗靶点。试剂盒检测范围为78 pg/ml- 5000 pg/ml,灵敏度为19.5 pg/ml。本试剂盒适用于体外实验中对GROα的定量分析,可服务于基础科研领域如炎症机制研究、肿瘤微环境模型构建、药物干预效果评估等方向,支持科研人员从动物模型、细胞培养或临床前样本中获取GROα的动态表达数据,为相关疾病的分子机制探索提供可靠工具。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 别名:
    C-X-C motif chemokine 1 ELISA Kit; Chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity; alpha) ELISA Kit; chemokine (C-X-C motif) ligand 1 ELISA Kit; CINC-1 ELISA Kit; CXCL1 ELISA Kit; Cytokine-induced neutrophil chemoattractant 1 ELISA Kit; Fibroblast secretory protein ELISA Kit; Fsp ELISA Kit; Gro 1 ELISA Kit; Gro A ELISA Kit; Gro ELISA Kit; GRO protein; alpha ELISA Kit; GRO-alpha(1-73) ELISA Kit; GRO-alpha(6-73) ELISA Kit; Gro1 ELISA Kit; GRO1 oncogene (melanoma growth stimulating activity; alpha) ELISA Kit; GRO1 oncogene (melanoma growth-stimulating activity) ELISA Kit; Gro1 oncogene ELISA Kit; GROa ELISA Kit; GROA_HUMAN ELISA Kit; Growth-regulated alpha protein ELISA Kit; KC ELISA Kit; KC chemokine; mouse; homolog of ELISA Kit; melanoma growth stimulatory activity alpha ELISA Kit; Melanoma growth stimulatory activity ELISA Kit; Melanoma growth stimulatory activity; alpha ELISA Kit; MGSA alpha ELISA Kit; MGSA ELISA Kit; MGSA-a ELISA Kit; N51 ELISA Kit; NAP-3 ELISA Kit; NAP3 ELISA Kit; Neutrophil-activating protein 3 ELISA Kit; Platelet-derived growth factor-inducible protein KC ELISA Kit; Scyb 1 ELISA Kit; Scyb1 ELISA Kit; Secretory protein N51 ELISA Kit; Small inducible cytokine subfamily B; member 1 ELISA Kit
  • 缩写:
    CXCL1
  • Uniprot No.:
    P09341
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    78 pg/ml - 5000 pg/ml
  • 灵敏度:
    19.5 pg/ml
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Immunology
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of human GROα/CXCL1/MGSA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    104


    Range %

    98-110


    1:2

    Average %

    97


    Range %

    91-105


    1:4

    Average %

    98


    Range %

    92-106


    1:8

    Average %

    93


    Range %

    86-98


  • 回收率:

    The recovery of human GROα/CXCL1/MGSA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    94

    89-99


    EDTA plasma (n=4)

    99

    92-108


  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    pg/ml

    OD1

    OD2

    Average

    Corrected


    5000

    2.543

    2.634

    2.589

    2.421


    2500

    2.135

    2.167

    2.151

    1.984


    1250

    1.574

    1.528

    1.551

    1.384


    625

    0.980

    0.959

    0.969

    0.802


    312

    0.550

    0.521

    0.536

    0.368


    156

    0.355

    0.343

    0.349

    0.182


    78

    0.260

    0.274

    0.267

    0.100


    0

    0.165

    0.170

    0.167




  • 货期:
    3-5 working days

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