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产品描述：人CX3C趋化因子受体1(CX3CR1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CX3CR1含量。CX3CR1是一种7次跨膜受体，主要介导CX3CL1的粘附和迁移功能，参与多种临床疾病的发病机制，如动脉粥样硬化、肾小球肾炎等。研究显示，CX3CR1通过调控巨噬细胞极化影响肾间质纤维化，其作用机制复杂，涉及细胞信号传导、免疫调节等多个方面。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，该产品适用于科研场景中CX3CR1蛋白表达水平的检测，例如探究其在神经炎症模型、心血管疾病机制或免疫细胞功能实验中的动态变化，为揭示CX3CR1在疾病发生发展中的调控作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CX3CR1; CMKBRL1; GPR13; CX3C chemokine receptor 1; C-X3-C CKR-1; CX3CR1; Beta chemokine receptor-like 1; CMK-BRL-1; CMK-BRL1; Fractalkine receptor; G-protein coupled receptor 13; V28
缩写：CX3CR1
Uniprot No.：P49238
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CX3CR1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 87 | |||||
| Range % | 82-92 | ||||||
| 1:2 | Average % | 92 | |||||
| Range % | 88-97 | ||||||
| 1:4 | Average % | 84 | |||||
| Range % | 80-88 | ||||||
| 1:8 | Average % | 101 | |||||
| Range % | 95-105 | ||||||
回收率：
| The recovery of human CX3CR1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 90-99 | |||||
| EDTA plasma (n=4) | 97 | 92-101 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected20002.7642.6472.7062.54910002.0062.0182.0121.8555001.2951.2381.2671.1102500.7310.7530.7420.5851250.4980.5120.5050.34862.50.3270.3410.3340.17731.250.2430.2390.2410.08400.1590.1540.157 | |||||||
货期：3-5 working days
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