| 纯度 | > 90 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | ARPC5 |
| Uniprot No | O15511 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-154aa |
| 氨基酸序列 | MSKNTVSSARFRKVDVDEYDENKFVDEEDGGDGQAGPDEGEVDSCLRHSITGNMTAALQAALKNPPINTKSQAVKDRAGSIVLKVLISFKANDIEKAVQSLDKNGVDLLMKYIYKGFESPSDNSSAMLLQWHEKALAAGGVGSIVRVLTARKTV |
| 预测分子量 | 43.6kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **文献名称**：*"Structural insights into the Arp2/3 complex and its interaction with actin filaments"*
**作者**：Robinson RC, et al.
**摘要**：该研究通过重组表达ARPC5及其他亚基，解析了Arp2/3复合体的三维结构，揭示了ARPC5在稳定复合体构象及调控肌动蛋白分支形成中的关键作用。
2. **文献名称**：*"Functional characterization of recombinant ARPC5 in cell migration and cancer metastasis"*
**作者**：Suraneni P, et al.
**摘要**：通过体外重组ARPC5蛋白实验，发现其缺失显著降低Arp2/3复合体的稳定性，并抑制肿瘤细胞的迁移和侵袭能力，表明ARPC5在癌症转移中的潜在治疗靶点价值。
3. **文献名称**：*"Expression and purification of the human Arp2/3 complex subunits for biochemical assays"*
**作者**：Goley ED, et al.
**摘要**：报道了利用大肠杆菌系统重组表达并纯化包括ARPC5在内的Arp2/3复合体亚基的方法，验证了重组蛋白在体外肌动蛋白聚合实验中的功能活性。
4. **文献名称**：*"ARPC5 isoforms regulate dendritic spine development in neurons"*
**作者**：Kim IH, et al.
**摘要**：研究比较了ARPC5两种异构体(ARPC5和ARPC5L)的重组蛋白功能，发现ARPC5特异性调控神经元树突棘形态发生，影响突触可塑性。
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注：以上文献信息为示例性内容，实际引用时需根据具体文献调整。
The ARPC5 (Actin-Related Protein 2/3 Complex Subunit 5) is a critical component of the Arp2/3 complex, a conserved seven-subunit protein assembly essential for regulating actin cytoskeleton dynamics. This complex facilitates actin nucleation and branching, enabling the formation of branched actin networks that drive cellular processes such as cell motility, membrane trafficking, and cytokinesis. ARPC5. along with ARPC4. forms the structural backbone of the Arp2/3 complex, stabilizing its conformation and mediating interactions with regulatory proteins like WASP/WAVE family activators.
Recombinant ARPC5 protein is produced via genetic engineering, typically expressed in *E. coli* or mammalian systems, followed by purification to ensure homogeneity. Its production enables detailed biochemical and structural studies to dissect Arp2/3 complex assembly, activation mechanisms, and interactions with actin or regulatory partners. Researchers use ARPC5 recombinant protein to investigate cytoskeletal remodeling defects linked to diseases, including cancer metastasis, immune cell dysfunction, and developmental disorders. For example, ARPC5 mutations or dysregulation have been implicated in impaired T-cell immune responses and invasive cancer phenotypes.
In drug discovery, recombinant ARPC5 serves as a tool for screening inhibitors targeting actin polymerization, potentially offering therapeutic strategies for conditions driven by aberrant cell migration. Its role in viral pathogenesis (e.g.,某些 viruses hijack Arp2/3 for intracellular transport) also makes it relevant in virology studies. Structural analyses, such as cryo-EM, often incorporate recombinant ARPC5 to resolve mechanistic details of Arp2/3-mediated actin branching. Overall, ARPC5 recombinant protein is a vital resource for advancing both basic research and applied biomedical studies related to cytoskeletal regulation.
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