首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人组织蛋白酶K(cath-K)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CTSK含量。CTSK，即组织蛋白酶K，是一种参与骨重塑和吸收的溶酶体半胱氨酸蛋白酶，主要在破骨细胞中表达。它通过有效降解骨细胞外基质，在骨吸收过程中发挥关键作用。CTSK的研究机制涉及其在骨代谢和甲状腺激素调节中的多方面功能，以及其在多种癌症中的作用，如促进肿瘤细胞增殖、侵袭和迁移。试剂盒检测范围为7.8 pg/mL-500 pg/mL，适用于骨代谢研究、肿瘤微环境分析、炎症性疾病机制探索等科研领域，通过检测不同实验条件下Cath-K的表达水平变化，为骨骼生物学、肿瘤侵袭转移及药物干预效果评估提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Cathepsin K ELISA Kit; Cathepsin O ELISA Kit; Cathepsin O1 ELISA Kit; Cathepsin O2 ELISA Kit; Cathepsin X ELISA Kit; CATK_HUMAN ELISA Kit; CTS02 ELISA Kit; Ctsk ELISA Kit; CTSO ELISA Kit; CTSO1 ELISA Kit; CTSO2 ELISA Kit; MGC23107 ELISA Kit; PKND ELISA Kit; PYCD ELISA Kit
缩写：CTSK
Uniprot No.：P43235
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：7.8 pg/mL-500 pg/mL
灵敏度：1.95 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human cath-K in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 92-105 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 88-98 | |||||
| 1:4 | Average % | 85 | ||||
| Range % | 80-90 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 91-100 | |||||
回收率：
| The recovery of human cath-K spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 90-102 | ||||
| EDTA plasma (n=4) | 95 | 88-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected5001.8861.8221.8541.7352501.5681.4891.5291.4101251.2131.2011.2071.08862.50.9760.9390.9580.83931.20.6520.6240.6380.51915.60.3480.3250.3370.2187.80.2060.1990.2030.08400.1210.1170.119 | ||||||
货期：3-5 working days
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