首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人组织蛋白酶D(cath-D)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、腹水样本中的CTSD含量。CTSD是一种重要靶点。它是一种溶酶体天冬氨酸蛋白酶，在多种生理过程中发挥作用。对其研究机制主要聚焦在它与细胞内蛋白降解、信号传导等方面的关联，还探索其在神经退行性疾病、癌症等疾病中的病理作用及潜在治疗价值。试剂盒检测范围为83 pg/mL-2000 pg/mL，科研人员可将其用于肿瘤微环境研究、细胞功能实验或疾病模型中蛋白酶活性变化的定量分析，为探索cath-D在癌症进展、神经退行性疾病等领域的分子机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CatD ELISA Kit; CATD_HUMAN ELISA Kit; Cathepsin D ELISA Kit; Cathepsin D heavy chain ELISA Kit; CD ELISA Kit; Ceroid lipofuscinosis neuronal 10 ELISA Kit; CLN10 ELISA Kit; CPSD ELISA Kit; ctsd ELISA Kit; Epididymis secretory sperm binding protein Li 130P ELISA Kit; HEL S 130P ELISA Kit; Lysosomal aspartyl peptidase ELISA Kit; Lysosomal aspartyl protease ELISA Kit; MGC2311 ELISA Kit
缩写：CTSD
Uniprot No.：P07339
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, ascitic fluid
检测范围：83 pg/mL-2000 pg/mL
灵敏度：33 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<10% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human cath-D in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 85-98 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 88-100 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 89-100 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 91-101 | |||||
回收率：
| The recovery of human cath-D spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 89-96 | ||||
| EDTA plasma (n=4) | 95 | 91-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.0041.9972.0011.95310001.3211.3561.3391.2915000.7850.8210.8030.7551670.3640.3410.3530.305830.2160.2290.2230.17500.0470.0480.048 | ||||||
货期：3-5 working days
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