首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人组织蛋白酶G(cath-G)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CTSG含量。CTSG为一种重要靶点。它在人体多种生理病理过程中有作用。研究发现其参与炎症反应、免疫调节等机制。深入探索CTSG的作用机制，有助于揭示相关疾病发病原理，为开发针对性治疗药物、制定治疗方案提供理论依据。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，研究者可借助探索中性粒细胞相关炎症反应机制、感染性疾病病理过程或免疫调控网络中的分子作用，为炎症性疾病、感染免疫及细胞信号转导等领域的机制研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CATG ELISA Kit; CATG_HUMAN ELISA Kit; Cathepsin G ELISA Kit; Cathepsin G precursor ELISA Kit; CathepsinG ELISA Kit; CG ELISA Kit; Ctsg ELISA Kit; CTSG protein ELISA Kit; MGC23078 ELISA Kit
缩写：CTSG
Uniprot No.：P08311
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human cath-G in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 92 | ||||
| Range % | 80-99 | |||||
| 1:10 | Average % | 97 | ||||
| Range % | 91-105 | |||||
| 1:20 | Average % | 97 | ||||
| Range % | 88-110 | |||||
| 1:40 | Average % | 93 | ||||
| Range % | 85-98 | |||||
回收率：
| The recovery of human cath-G spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 96 | 91-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.6172.6542.6362.51210002.2052.2542.2302.1065001.6361.7051.6711.5472500.9430.9890.9660.8421250.5090.5540.5320.40862.50.3520.3690.3610.23731.250.2260.2410.2340.11000.1220.1260.124 | ||||||
货期：3-5 working days
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