首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人胱抑素F(CST7)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的CST7含量。试剂盒检测范围为0.625 ng/mL-40 ng/mL，适用于科研场景中CST7的功能研究，例如探究其在炎症反应、肿瘤侵袭或神经病变模型中的表达变化规律，或通过体外细胞培养实验分析特定刺激下CST7的分泌动态，为疾病机制研究及潜在生物标志物探索提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CMAP ELISA Kit; CST 7 ELISA Kit; Cst7 ELISA Kit; Cystatin 7 ELISA Kit; cystatin F (leukocystatin) ELISA Kit; Cystatin like metastasis associated protein ELISA Kit; Cystatin-7 ELISA Kit; Cystatin-F ELISA Kit; Cystatin-like metastasis-associated protein ELISA Kit; Cystatin7 ELISA Kit; CystatinF ELISA Kit; CYTF_HUMAN ELISA Kit; dJ568C11.1 ELISA Kit; Leukocystatin ELISA Kit
缩写：CST7
Uniprot No.：O76096
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CST7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 93 | |||||
| Range % | 85-97 | ||||||
| 1:2 | Average % | 98 | |||||
| Range % | 91-101 | ||||||
| 1:4 | Average % | 94 | |||||
| Range % | 85-97 | ||||||
| 1:8 | Average % | 90 | |||||
| Range % | 84-95 | ||||||
回收率：
| The recovery of human CST7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 100 | 94-107 | |||||
| EDTA plasma (n=4) | 106 | 98-112 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected402.3192.2352.2772.124201.6851.6711.6781.525100.9780.9270.9530.80050.6410.6270.6340.4812.50.3550.3690.3620.2091.250.2470.2600.2540.1010.6250.1950.1880.1920.03900.1510.1550.153 | |||||||
货期：3-5 working days
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