首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人胱抑素SN(CST1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液,尿液、组织匀浆、细胞裂解物样本中的CST1含量。CST1（胱抑素C）是一种分泌蛋白，属于胱抑素家族，具有抑制组织蛋白酶活性的功能。在肿瘤生长、血管生成、浸润和转移中起重要作用。通过生物信息学分析，研究人员揭示了CST1在乳腺癌等癌症中的潜在作用机制，为癌症诊断和治疗提供了新的靶点。试剂盒检测范围为0.156 ng/mL-10 ng/mL，科研领域可用于探究CST1在黏膜防御机制、炎症相关信号通路或肿瘤微环境中的生物学功能，也可作为研究慢性炎症性疾病、黏膜屏障功能障碍或肿瘤标志物筛选的工具，为疾病机制研究提供数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CST1 ELISA Kit; Cystain SAI ELISA Kit; Cystain-SA-I ELISA Kit; Cystatin 1 ELISA Kit; Cystatin-1 ELISA Kit; Cystatin-SN ELISA Kit; Cystatin1 ELISA Kit; Cysteine proteinase inhibitor, type 2 family ELISA Kit; CYTN ELISA Kit; CYTN_HUMAN ELISA Kit; Salivary cystatin SA1 ELISA Kit; Salivary cystatin-SA-1 ELISA Kit
缩写：CST1
Uniprot No.：P01037
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates,urine, tissue homogenates, cell lysates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CST1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 92-97 | |||||
| 1:2 | Average % | 100 | ||||
| Range % | 98-104 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 92-101 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human CST1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 92-100 | ||||
| EDTA plasma (n=4) | 93 | 90-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 102.4062.3892.3982.278 51.8411.7761.8091.689 2.51.2461.2231.2351.115 1.250.6950.6850.6900.570 0.6250.4640.4440.4540.334 0.3120.2520.2490.2510.131 0.1560.1870.1850.1860.066 00.1220.1180.120 | ||||||
货期：3-5 working days
×
