首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人硫酸软骨素蛋白多糖4(CSPG4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CSPG4含量。CSPG4即硫酸软骨素蛋白聚糖4，是一种跨膜蛋白，在多种恶性肿瘤中高表达，与肿瘤细胞的增殖、迁移、侵袭等密切相关。目前其作为肿瘤治疗靶点的研究较多，机制上主要是通过阻断相关信号通路、激活免疫反应等来发挥抗肿瘤作用。试剂盒检测范围为25 pg/mL-1600 pg/mL，科研人员可通过探索CSPG4在肿瘤生物学行为、干细胞分化调控或炎症反应中的分子机制，亦可用于评估疾病模型动物组织或体外培养细胞中CSPG4的表达水平变化，为相关基础研究及转化医学提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：4732461B14Rik ELISA Kit; AN2 ELISA Kit; AN2 proteoglycan ELISA Kit; Chondroitin sulfate proteoglycan 4 (melanoma-associated) ELISA Kit; Chondroitin sulfate proteoglycan 4 ELISA Kit; Chondroitin sulfate proteoglycan NG2 ELISA Kit; Cspg4 ELISA Kit; Cspg4 chondroitin sulfate proteoglycan 4 ELISA Kit; CSPG4_HUMAN ELISA Kit; HMW-MAA ELISA Kit; HSN tumor-specific antigen ELISA Kit; Kiaa4232 ELISA Kit; MCSP ELISA Kit; MCSPG ELISA Kit; MEL-CSPG ELISA Kit; Melanoma chondroitin sulfate proteoglycan ELISA Kit; Melanoma-associated chondroitin sulfate proteoglycan ELISA Kit; MELCSPG ELISA Kit; MSK16 ELISA Kit; NG2 ELISA Kit
缩写：CSPG4
Uniprot No.：Q6UVK1
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：25 pg/mL-1600 pg/mL
灵敏度：6.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CSPG4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 87-95 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 83-94 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 89-95 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 92-103 | |||||
回收率：
| The recovery of human CSPG4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 91-102 | ||||
| EDTA plasma (n=4) | 88 | 82-94 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected16002.1702.1342.1521.9588001.5011.6021.5521.3584001.0111.0891.0500.8562000.7370.7250.7310.5371000.4740.4630.4690.275500.3410.3550.3480.154250.2630.2730.2680.07400.1930.1940.194 | ||||||
货期：3-5 working days
×
