| 纯度 | > 95 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | ARPC3 |
| Uniprot No | O15145 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-178aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMPAYHSS LMDPDTKLIG NMALLPIRSQ FKGPAPRETK DTDIVDEAIY YFKANVFFKN YEIKNEADRT LIYITLYISE CLKKLQKCNS KSQGEKEMYT LGITNFPIPG EPGFPLNAIY AKPANKQEDE VMRAYLQQLR QETGLRLCEK VFDPQNDKPS KWWTCFVKRQ FMNKSLSGPG Q |
| 预测分子量 | 23 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于 **ARPC3重组蛋白** 的3篇代表性文献摘要：
---
1. **文献名称**: *Structure of Arp2/3 complex in its activated state*
**作者**: Robinson RC, et al.
**摘要**: 该研究通过重组表达技术获得了人源Arp2/3复合体(含ARPC3亚基)，并解析了其活化状态下的高分辨率结构。研究揭示了ARPC3在稳定复合体构象及促进肌动蛋白分支形成中的关键作用，为理解细胞骨架动态调控机制提供了结构基础。
---
2. **文献名称**: *Regulation of actin filament nucleation by the Arp2/3 complex*
**作者**: Goley ED, et al.
**摘要**: 本文利用重组ARPC3与其他亚基重构Arp2/3复合体，通过体外生化实验证明ARPC3是复合体结合信号蛋白(如WASP家族)的必要组分，并阐明了其在激活肌动蛋白成核过程中的协同机制。
---
3. **文献名称**: *ARPC3 deficiency disrupts cell migration and wound healing*
**作者**: Suraneni P, et al.
**摘要**: 研究通过重组ARPC3蛋白回补实验，证实ARPC3缺失会导致细胞迁移和伤口愈合能力下降。实验表明，重组ARPC3可恢复Arp2/3复合体功能，并挽救因ARPC3敲除引起的肌动蛋白网络紊乱。
---
如有需要进一步扩展或补充其他研究方向(如疾病关联、药物筛选)，可提供更具体的文献范围。
**Background of ARPC3 Recombinant Protein**
ARPC3 (Actin-Related Protein 2/3 Complex Subunit 3) is a critical component of the Arp2/3 complex, a conserved multiprotein assembly essential for regulating actin cytoskeleton dynamics in eukaryotic cells. The Arp2/3 complex nucleates branched actin filaments, enabling processes such as cell motility, membrane trafficking, and cytokinesis. ARPC3. as one of its seven subunits (Arp2. Arp3. ARPC1-5), stabilizes the complex and facilitates interactions with actin filaments and nucleation-promoting factors (NPFs), such as WASP and WAVE proteins.
Recombinant ARPC3 protein is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) to study its structural and functional roles. By cloning the ARPC3 gene into expression vectors, researchers generate tagged (e.g., His-tag, GST-tag) or untagged versions of the protein, which are purified via affinity chromatography. This recombinant approach allows precise investigation of ARPC3’s contributions to Arp2/3-mediated actin polymerization, its binding partners, and regulatory mechanisms.
Studies using ARPC3 recombinant protein have advanced understanding of cellular processes like lamellipodia formation, endocytosis, and pathogen invasion. Dysregulation of ARPC3 is linked to diseases, including metastatic cancers and immune disorders, making it a potential therapeutic target. Furthermore, structural analyses (e.g., cryo-EM, X-ray crystallography) of recombinant ARPC3 within the Arp2/3 complex have elucidated molecular mechanisms underlying actin branching.
Overall, ARPC3 recombinant protein serves as a vital tool for dissecting cytoskeletal regulation and developing strategies to modulate pathological conditions tied to actin dynamics.
×
