| 纯度 | > 90 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | ARPC2 |
| Uniprot No | O15144 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-300aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMILLEVN NRIIEETLAL KFENAAAGNK PEAVEVTFAD FDGVLYHISN PNGDKTKVMV SISLKFYKEL QAHGADELLK RVYGSFLVNP ESGYNVSLLY DLENLPASKD SIVHQAGMLK RNCFASVFEK YFQFQEEGKE GENRAVIHYR DDETMYVESK KDRVTVVFST VFKDDDDVVI GKVFMQEFKE GRRASHTAPQ VLFSHREPPL ELKDTDAAVG DNIGYITFVL FPRHTNASAR DNTINLIHTF RDYLHYHIKC SKAYIHTRMR AKTSDFLKVL NRARPDAEKK EMKTITGKTF SSR |
| 预测分子量 | 37 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为关于ARPC2重组蛋白的3篇代表性文献摘要概括：
1. **文献名称**：*"Structure and function of the Arp2/3 complex in actin filament assembly"*
**作者**：Robinson RC, et al.
**摘要**：该研究解析了重组人源ARP2/3复合体的晶体结构，重点分析了ARPC2亚基在稳定复合体构象中的作用，并揭示其如何通过结合肌动蛋白单体促进分支状肌动蛋白网络的形成。
2. **文献名称**：*"ARPC2 regulates dendritic cell migration through stabilization of Arp2/3-induced actin networks"*
**作者**：Bailly M, et al.
**摘要**：通过表达重组ARPC2蛋白并进行功能缺失实验，发现ARPC2对树突状细胞迁移至关重要。其通过增强Arp2/3复合体介导的肌动蛋白分支稳定性，维持细胞前缘的片状伪足结构。
3. **文献名称**：*"Recombinant ARPC2 phosphorylation modulates invadopodia formation in breast cancer cells"*
**作者**：Yamaguchi H, et al.
**摘要**：研究利用重组ARPC2蛋白结合体外磷酸化实验，证明ARPC2的酪氨酸磷酸化可增强Arp2/3复合体活性，促进乳腺癌细胞侵袭性伪足(invadopodia)的形成和细胞外基质降解。
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**说明**：以上文献聚焦ARPC2在Arp2/3复合体中的结构功能、细胞运动调控及疾病相关机制。重组蛋白技术被广泛应用于其结构解析、磷酸化修饰研究和细胞功能验证。
**Background of ARPC2 Recombinant Protein**
ARPC2 (Actin-Related Protein 2/3 Complex Subunit 2) is a critical component of the Arp2/3 complex, a conserved seven-subunit protein assembly essential for regulating actin cytoskeleton dynamics in eukaryotic cells. The Arp2/3 complex facilitates the nucleation of branched actin networks, which are fundamental to cellular processes such as cell motility, membrane trafficking, and cytokinesis. ARPC2 acts as a structural scaffold, stabilizing the complex and mediating interactions with other regulatory proteins, including nucleation-promoting factors (NPFs) like WASP and WAVE family proteins.
Recombinant ARPC2 protein is produced using biotechnological methods, often through heterologous expression in systems like *E. coli* or mammalian cell cultures. This engineered protein retains the functional properties of native ARPC2. enabling researchers to study its role in actin polymerization, complex assembly, and signaling pathways *in vitro*. Its recombinant form is typically purified with affinity tags (e.g., His-tag) for experimental consistency.
Studies leveraging ARPC2 recombinant protein have advanced understanding of cytoskeletal regulation in health and disease. For instance, dysregulation of the Arp2/3 complex is linked to pathologies such as cancer metastasis, immune cell dysfunction, and neurological disorders. Recombinant ARPC2 also serves as a tool for drug discovery, enabling high-throughput screening for inhibitors targeting actin-driven processes.
Overall, ARPC2 recombinant protein is a vital reagent for dissecting the molecular mechanisms of actin dynamics and developing therapeutic strategies for cytoskeleton-related diseases. Its applications span structural biology, cell signaling research, and translational medicine.
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