首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人羧肽酶E(CPE)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CPE含量。CPE即羧肽酶E，是一种与神经内分泌相关的酶。其背景在于在脑和内分泌组织中广泛存在。研究机制上，它参与神经肽和激素前体加工，对调节神经递质释放、内分泌信号传导等有重要作用，在神经生理和内分泌相关疾病研究方面受关注。试剂盒检测范围为39.06 pg/mL-2500 pg/mL，适用于科研场景下探究CPE在代谢性疾病模型、神经内分泌调控机制中的表达变化，亦可用于评估药物干预或基因修饰对CPE活性的影响。为研究CPE在病理生理过程中的分子机制及其作为潜在生物标志物的探索提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Carboxypeptidase E ELISA Kit; Carboxypeptidase H ELISA Kit; Carboxypeptidase H precursor ELISA Kit; CarboxypeptidaseE ELISA Kit; CarboxypeptidaseH ELISA Kit; CBPE_HUMAN ELISA Kit; Cobalt stimulated chromaffin granule carboxypeptidase ELISA Kit; CPE ELISA Kit; Cph 1 ELISA Kit; CPH ELISA Kit; Enkephalin convertase ELISA Kit; Entephalin convertase ELISA Kit; Insulin granule associated carboxypeptidase ELISA Kit; Prohormone processing carboxypeptidase ELISA Kit; Prohormone-processing carboxypeptidase ELISA Kit
缩写：CPE
Uniprot No.：P16870
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：39.06 pg/mL-2500 pg/mL
灵敏度：9.76 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CPE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 105 | |||||
| Range % | 94-109 | ||||||
| 1:2 | Average % | 107 | |||||
| Range % | 98-111 | ||||||
| 1:4 | Average % | 92 | |||||
| Range % | 84-95 | ||||||
| 1:8 | Average % | 98 | |||||
| Range % | 90-102 | ||||||
回收率：
| The recovery of human CPE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 91 | 84-94 | |||||
| EDTA plasma (n=4) | 101 | 95-104 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected25002.4102.3332.3722.24612501.7121.6791.6961.5706251.0290.9680.9990.873312.50.6340.6550.6450.519156.250.4100.4010.4060.28078.120.2800.2840.2820.15639.060.2180.2250.2220.09600.1250.1270.126 | |||||||
货期：3-5 working days
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