首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人肥大细胞类羧肽酶酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CPA3含量。CPA3是一个值得关注的靶点。它在人体生理与病理过程中发挥作用。目前研究聚焦于其参与的免疫反应、炎症调节等机制。了解其作用机制有助于深入认识相关疾病发病原理，为开发针对性治疗手段如药物研发等提供重要方向。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于科研领域中对肥大细胞相关疾病机制的研究，如过敏性疾病模型构建、炎症标志物分析以及药物干预下CPA3表达水平变化的评估，为探索肥大细胞功能调控提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：carboxypeptidase A ELISA Kit; carboxypeptidase A3 (mast cell) ELISA Kit; Carboxypeptidase A3 ELISA Kit; carboxypeptidase A3 mast cell ELISA Kit; CBPA3_HUMAN ELISA Kit; Cpa3 ELISA Kit; Mast cell carboxypeptidase A ELISA Kit; mast cell carboxypeptidase A3 ELISA Kit; MC CPA ELISA Kit; MC-CPA ELISA Kit; tissue carboxypeptidase A ELISA Kit
缩写：CPA3
Uniprot No.：P15088
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human MC-CPA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 80-92 | |||||
| 1:2 | Average % | 100 | ||||
| Range % | 94-103 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 94-100 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human MC-CPA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-98 | ||||
| EDTA plasma (n=4) | 96 | 94-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.4772.4532.4652.366101.9931.9241.9591.86051.2801.2711.2761.1772.50.6850.6780.6820.5831.250.4330.4240.4290.3300.6250.2860.2690.2780.1790.3120.1900.1790.1850.08600.1010.0970.099 | ||||||
货期：3-5 working days
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