首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人铜蓝蛋白(CP/CER)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、尿液、唾液样本中的CP含量。人铜蓝蛋白（CP/CER）是一种含铜的α2糖蛋白，在许多疾病中其含量会发生变化，受到广泛关注。其分子组成含铜约0.27～0.34%，具有氧化酶活性，参与铜离子运输和抗氧化作用，与多种疾病如肝豆状核变性相关。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于科研领域中对铜代谢相关疾病的机制探索、细胞模型中铜蓝蛋白分泌水平的动态监测，以及生物体液样本的蛋白质组学分析，为体外实验提供可靠的定量检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CERU_HUMAN ELISA Kit; Ceruloplasmin ELISA Kit; CP 2 ELISA Kit; CP ELISA Kit; CP2 ELISA Kit; Ferroxidase ELISA Kit
缩写：CP
Uniprot No.：P00450
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, urine, saliva
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:50000 | Average % | 93 | ||||
| Range % | 90-98 | |||||
| 1:100000 | Average % | 92 | ||||
| Range % | 87-97 | |||||
| 1:200000 | Average % | 95 | ||||
| Range % | 91-103 | |||||
| 1:400000 | Average % | 94 | ||||
| Range % | 86-100 | |||||
回收率：
| The recovery of human CP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 89-102 | ||||
| EDTA plasma (n=4) | 93 | 88-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.7502.8162.7832.62552.1512.1142.1331.9752.51.3681.3901.3791.2211.250.8760.9110.8940.7360.6250.5450.5680.5570.3990.3120.3820.4050.3940.2360.1560.2810.2890.2850.12700.1580.1570.158 | ||||||
货期：3-5 working days
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