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产品描述：人胶原凝集素-10(COLEC10)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的COLEC10含量。COLEC10是一种C型凝集素家族成员，主要在肝星状细胞中表达，参与肝纤维化的发病机制。其功能涉及免疫反应和炎症过程，可能通过识别病原体相关分子模式（PAMP）和损伤相关分子模式（DAMP）来激活免疫细胞，增强宿主对感染的防御。试剂盒检测范围为23.44 pg/mL-1500 pg/mL，适用于细胞生物学、免疫学及疾病机制研究中COLEC10的表达动态分析，例如炎症模型构建、病原体感染应答研究或肿瘤相关分子网络探索。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CL-34 ELISA Kit; CL-L1 ELISA Kit; CLL1 ELISA Kit; COL10_HUMAN ELISA Kit; Colec10 ELISA Kit; Collectin liver 1 ELISA Kit; Collectin liver protein 1 ELISA Kit; Collectin sub family member 10 (C-type lectin) ELISA Kit; Collectin sub family member 10 ELISA Kit; Collectin subfamily member 10 ELISA Kit; Collectin-10 ELISA Kit; Collectin-34 ELISA Kit; MGC118794 ELISA Kit; MGC118795 ELISA Kit; OTTHUMP00000228058 ELISA Kit
缩写：COLEC10
Uniprot No.：Q9Y6Z7
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：23.44 pg/mL-1500 pg/mL
灵敏度：5.86 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human COLEC10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 81-92 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 83-98 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 93-101 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 87-99 | |||||
回收率：
| The recovery of human COLEC10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 92-102 | ||||
| EDTA plasma (n=4) | 88 | 82-94 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected15002.8802.9642.9222.8147502.5412.4272.4842.3763751.8321.7341.7831.675187.50.9970.9720.9850.87793.750.5330.5540.5440.43646.880.3800.3760.3780.27023.440.2540.2580.2560.14800.1070.1080.108 | ||||||
货期：3-5 working days
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