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产品描述：人VI型胶原α-3链(COL6A3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的COL6A3含量。人VI型胶原α-3链（COL6A3）是编码VI型胶原蛋白α-3链的基因，存在于大多数结缔组织中，其突变与多种肌肉疾病相关，如贝特莱姆肌病和乌尔里希先天性肌营养不良症。此外，COL6A3在结直肠癌、胰腺癌、卵巢癌和胃癌中表达升高，可能与肿瘤的发生发展有关。试剂盒检测范围为1.56 ng/mL-100 ng/mL，适用于多种生物样本中COL6A3的定量分析，尤其适合研究肌肉组织修复机制、纤维化疾病模型或细胞外基质动态变化等科研场景。本试剂盒为研究COL6A3在组织工程、疾病模型构建及分子机制探索中的应用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CO6A3_HUMAN ELISA Kit; COL6A3 ELISA Kit; Collagen alpha-3(VI) chain ELISA Kit; Collagen type VI alpha 3 ELISA Kit; Collagen VI alpha 3 ELISA Kit
缩写：COL6A3
Uniprot No.：P12111
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human COL6A3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 86 | |||||
| Range % | 80-89 | ||||||
| 1:2 | Average % | 91 | |||||
| Range % | 87-95 | ||||||
| 1:4 | Average % | 92 | |||||
| Range % | 87-98 | ||||||
| 1:8 | Average % | 93 | |||||
| Range % | 86-97 | ||||||
回收率：
| The recovery of human COL6A3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 87 | 82-91 | |||||
| EDTA plasma (n=4) | 102 | 97-106 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected1001.9881.8721.9301.821501.1921.2621.2271.118250.7240.7690.7470.63812.50.4510.4670.4590.3506.250.3040.3140.3090.2003.120.2170.2220.2200.1111.560.1600.1690.1650.05600.1080.1090.109 | |||||||
货期：3-5 working days
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