首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人VII型胶原α-1链(COL7A1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的COL7A1含量。COL7A1基因编码Ⅶ型胶原蛋白，其在维持皮肤及其他组织的结构完整性方面发挥重要作用。研究发现它的突变可引发遗传性大疱性表皮松解症。机制上，聚焦于理解其在基底膜带形成及锚定纤维构建中的功能，为相关疾病治疗提供靶点。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于体外研究皮肤基底膜复合物形成机制、遗传性表皮松解动物模型评估、以及皮肤组织工程中胶原代谢相关研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Alpha 1 type VII collagen ELISA Kit; CO7A1_HUMAN ELISA Kit; Col7a1 ELISA Kit; Collagen 7 ELISA Kit; Collagen alpha 1(VII) chain ELISA Kit; Collagen alpha-1(VII) chain ELISA Kit; Collagen type VII alpha 1 ELISA Kit; Collagen VII alpha 1 polypeptide ELISA Kit; CollagenVII ELISA Kit; EBD 1 ELISA Kit; EBD1 ELISA Kit; EBDCT ELISA Kit; EBR 1 ELISA Kit; EBR1 ELISA Kit; LC collagen ELISA Kit; Long chain collagen ELISA Kit; Long-chain collagen ELISA Kit
缩写：COL7A1
Uniprot No.：Q02388
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human COL7A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 88-93 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 95-101 | |||||
| 1:4 | Average % | 102 | ||||
| Range % | 97-105 | |||||
| 1:8 | Average % | 89 | ||||
| Range % | 82-96 | |||||
回收率：
| The recovery of human COL7A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 89-95 | ||||
| EDTA plasma (n=4) | 101 | 98-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.4542.6542.5542.392102.0752.2222.1491.98751.4391.5401.4901.3282.50.8790.8570.8680.7061.250.6250.6480.6370.4750.6250.4090.4440.4270.2650.3120.2920.2990.2960.13400.1570.1660.162 | ||||||
货期：3-5 working days
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