首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人Ⅵ型胶原(COLⅥ)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、尿液样本中的COL6A1含量。人Ⅵ型胶原（COLⅥ）是一种细胞外基质蛋白，存在于结缔组织中，与心血管疾病、肌肉疾病和肝纤维化等密切相关。其功能包括维持组织完整性、促进细胞增殖和迁移。研究显示，COLⅥ的基因突变可导致Ullrich先天性肌营养不良和Bethlem肌病。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，该产品可为纤维化疾病机制研究、再生医学中组织修复评估、肿瘤微环境分析以及泌尿系统相关研究提供可靠的数据支持，尤其适用于体外实验模型中COLⅥ表达水平的定量监测。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Alpha 1 (VI) chain (61 AA) ELISA Kit; CO6A1_HUMAN ELISA Kit; COL6A1 ELISA Kit; COL6A2 ELISA Kit; COL6A3 ELISA Kit; collagen 6 ELISA Kit; Collagen alpha 2(VI) chain ELISA Kit; Collagen alpha 3(VI) chain ELISA Kit; Collagen alpha-1(VI) chain ELISA Kit; collagen six ELISA Kit; Collagen type VI alpha 1 ELISA Kit; Collagen type VI alpha 2 ELISA Kit; Collagen type VI alpha 3 ELISA Kit; Collagen VI alpha 1 polypeptide ELISA Kit; Collagen VI alpha 2 polypeptide ELISA Kit; Collagen VI alpha 3 polypeptide ELISA Kit; CollagenVI ELISA Kit; Human mRNA for collagen VI alpha 2 C terminal globular domain ELISA Kit; OPLL ELISA Kit; PP3610 ELISA Kit
缩写：COL6A1
Uniprot No.：P12109
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, urine
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human COLVI in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:200 | Average % | 86 | ||||
| Range % | 80-96 | |||||
| 1:400 | Average % | 97 | ||||
| Range % | 91-98 | |||||
| 1:800 | Average % | 98 | ||||
| Range % | 92-105 | |||||
| 1:1600 | Average % | 102 | ||||
| Range % | 86-110 | |||||
回收率：
| The recovery ofhuman COLVI spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 89-110 | ||||
| EDTA plasma (n=4) | 96 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.9122.8122.8622.70210002.1372.1032.1201.9605001.1561.0981.1270.9672500.6140.5780.5960.4361250.3720.3460.3590.19962.50.2810.2790.2800.12031.250.2020.1980.2000.04000.1610.1590.160 | ||||||
货期：3-5 working days
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