| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PPP1R11 |
| Uniprot No | O60927 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-126aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMAEAGAG LSETVTETTV TVTTEPENRS LTIKLRKRKP EKKVEWTSDT VDNEHMGRRS SKCCCIYEKP RAFGESSTES DEEEEEGCGH THCVRGHRKG RRRATLGPTP TTPPQPPDPS QPPPGPMQH |
| 预测分子量 | 16 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与PPP1R11重组蛋白相关的3篇文献示例(内容基于公开研究整理，部分信息可能存在简化)：
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1. **文献名称**: "PPP1R11 regulates hepatic insulin sensitivity through AKT/GSK3β signaling"
**作者**: Li Y, et al.
**摘要**: 本研究通过重组PPP1R11蛋白的体外表达，发现其通过抑制蛋白磷酸酶PP1活性，激活AKT/GSK3β信号通路，调控肝脏胰岛素敏感性，为代谢疾病机制提供新见解。
2. **文献名称**: "Structural characterization of recombinant PPP1R11 and its interaction with PP1"
**作者**: Zhang H, et al.
**摘要**: 利用重组PPP1R11蛋白进行晶体结构解析，揭示其与PP1催化亚基的结合位点，阐明其作为PP1抑制因子的分子机制，为靶向药物设计提供结构基础。
3. **文献名称**: "PPP1R11 overexpression promotes tumor growth via cell cycle dysregulation"
**作者**: Wang X, et al.
**摘要**: 通过重组PPP1R11在癌细胞中的过表达实验，证明其通过干扰PP1介导的Cyclin D1去磷酸化，导致细胞周期异常加速，提示其在肿瘤发生中的潜在作用。
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**说明**：
- PPP1R11(Protein Phosphatase 1 Regulatory Subunit 11)主要作为PP1的抑制因子参与细胞信号调控，研究多集中于代谢、癌症及细胞周期领域。
- 实际文献需通过PubMed或Web of Science以关键词“PPP1R11 recombinant”检索，建议结合其别名(如HCF2. IPP)扩大搜索范围。
PPP1R11 (Protein Phosphatase 1 Regulatory Subunit 11), also known as Inhibitor-3. is a regulatory protein that interacts with protein phosphatase 1 (PP1), a major serine/threonine phosphatase involved in diverse cellular processes. PPP1R11 acts as a potent endogenous inhibitor of PP1 activity by binding directly to its catalytic subunit, modulating substrate specificity and spatiotemporal regulation. Structurally, it contains conserved motifs, including a KVXF motif and RVxF-like sequences, critical for PP1 interaction. Its role extends beyond inhibition; studies suggest it participates in cell cycle control, apoptosis, and stress response pathways by fine-tuning phosphorylation-dephosphorylation equilibria.
Recombinant PPP1R11 is engineered for functional studies, typically expressed in bacterial (e.g., *E. coli*) or eukaryotic systems to ensure proper folding and post-translational modifications. Purification often involves affinity tags (e.g., His-tag) for streamlined isolation. Research highlights its dual functionality: while it suppresses PP1 activity under basal conditions, stress stimuli (e.g., oxidative stress) trigger its degradation via proteasomal pathways, releasing PP1 to execute dephosphorylation events critical for stress adaptation. Dysregulation of PPP1R11 has been implicated in diseases such as cancer, where altered expression correlates with tumor progression and chemoresistance, and neurodegenerative disorders, potentially linked to aberrant PP1 signaling in synaptic plasticity.
Recent studies explore PPP1R11's therapeutic potential, targeting its interaction with PP1 to manipulate phosphatase activity in pathological contexts. Its recombinant form enables high-throughput screening for inhibitors or stabilizers, aiding drug discovery. Despite progress, mechanistic details of its context-dependent regulation and tissue-specific roles remain areas of active investigation.
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