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产品描述：人III型胶原α1(COL3A1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的COL3A1含量。COL3A1编码Ⅲ型胶原蛋白的α1链，在维持组织弹性和韧性等方面发挥重要作用。研究发现它与肿瘤进展、纤维化疾病等密切相关，机制上可能通过影响细胞外基质重塑、细胞黏附迁移等参与疾病进程，有望成为相关疾病治疗新靶点。试剂盒检测范围为0.156 ng/mL-10 ng/mL，该产品适用于基础科研领域，如纤维化疾病模型研究、肿瘤侵袭转移机制探索或干细胞分化过程中基质重塑分析，为细胞外基质动态变化研究提供标准化工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Alpha 1 type III collagen ELISA Kit; Alpha1 (III) collagen ELISA Kit; CO3A1_HUMAN ELISA Kit; COL 3A1 ELISA Kit; COL3A1 ELISA Kit; Collagen alpha 1(III) chain ELISA Kit; Collagen alpha-1(III) chain ELISA Kit; Collagen III alpha 1 chain precursor ELISA Kit; Collagen III alpha 1 polypeptide ELISA Kit; Collagen type III alpha 1 (Ehlers Danlos syndrome type IV autosomal dominant) ELISA Kit; Collagen type III alpha 1 ELISA Kit; Collagen type III alpha 1 chain ELISA Kit; Collagen type III alpha ELISA Kit; Collagen; fetal ELISA Kit; EDS4A ELISA Kit; Ehlers Danlos syndrome type IV; autosomal dominant ELISA Kit; Fetal collagen ELISA Kit; Type III collagen ELISA Kit
缩写：COL3A1
Uniprot No.：P02461
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human COL3A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 85-96 | |||||
| 1:2 | Average % | 100 | ||||
| Range % | 95-106 | |||||
| 1:4 | Average % | 101 | ||||
| Range % | 95-106 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 90-107 | |||||
回收率：
| The recovery of human COL3A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 85-98 | ||||
| EDTA plasma (n=4) | 109 | 102-115 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 102.4842.5752.5302.381 51.7881.8891.8391.690 2.51.2561.3651.3111.162 1.250.7320.7660.7490.600 0.6250.4170.4500.4340.285 0.3120.2740.2840.2790.130 0.1560.2480.2500.2490.100 00.1460.1510.149 | ||||||
本试剂盒所含材料：
A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human COL3A1 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled COL3A1 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibodyDiluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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