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产品描述：人II型胶原α-1链(COL2A1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的COL2A1含量。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于基础科研领域，包括但不限于软骨代谢调控机制研究、退行性关节疾病动物模型验证、生物工程材料对软骨细胞功能影响的体外评估，以及药物开发中靶向软骨修复的活性物质筛选。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Alpha 1 type II collagen ELISA Kit; Alpha-1 type II collagen ELISA Kit; AOM ELISA Kit; Cartilage collagen ELISA Kit; Chondrocalcin ELISA Kit; CO2A1_HUMAN ELISA Kit; COL11A3 ELISA Kit; Col2a1 ELISA Kit; Collagen 2 ELISA Kit; Collagen II alpha 1 polypeptide ELISA Kit; SEDC ELISA Kit
缩写：COL2A1
Uniprot No.：P02458
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human COL2A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 104 | |||||
| Range % | 100-107 | ||||||
| 1:2 | Average % | 85 | |||||
| Range % | 81-89 | ||||||
| 1:4 | Average % | 96 | |||||
| Range % | 93-99 | ||||||
| 1:8 | Average % | 88 | |||||
| Range % | 84-93 | ||||||
回收率：
| The recovery of human COL2A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 100 | 95-105 | |||||
| EDTA plasma (n=4) | 102 | 98-106 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected202.3052.2052.2552.092101.9631.9241.9441.78151.6141.6311.6231.4602.51.2041.3041.2541.0911.250.7820.7980.7900.6270.6250.4630.4760.4700.3070.3120.3060.3120.3090.14600.1620.1640.163 | |||||||
货期：3-5 working days
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